Lai Tung-yuan, Weng Yi-jiun, Kuo Wei-wen, Chen Li-mien, Chung Yun-ting, Lin Yueh-min, Tsai Fuu-jen, Lee Chun-hsin, Choong Yee-mun, Lai Eric Y C, Huang Chih-yang, Yeh Yu-lan
School of Post-Baccalaureate Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung 404, Taiwan, China.
Zhong Xi Yi Jie He Xue Bao. 2010 Jan;8(1):49-55. doi: 10.3736/jcim20100110.
To clarify the efficacy of Taohe Chengqi Tang (THCQT), a compound traditional Chinese herbal medicine, in protecting liver damage induced by carbon tetrachloride (CCl(4)) in rats.
Thirty male Wistar rats were divided into normal control group, untreated group, low-dose THCQT group (receiving 0.3 g/kg of THCQT), high-dose THCQT group (receiving 0.5 g/kg of THCQT), and positive control group (receiving silymarin 25 mg/kg). All testing substances were orally administered 1 hour before the intraperitoneal injection of CCl(4) (1.5 mL/kg). Twenty-four hours after CCl(4) injection, the rats were sacrificed to observe liver histopathological changes, and to evaluate activities of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT), and lipid peroxidation (LPO) and glutathione (GSH) levels in liver tissues.
CCl(4) injection elevated the serum AST and ALT activities, but THCQT significantly reversed this effect. The increase of hepatic LPO by CCl(4) was markedly reduced by THCQT. Also, this herbal mixture increased hepatic GSH in the rats. In histopathology analysis, THCQT decreased the fatty accumulation, necrosis and lymphocyte infiltration. The in vitro study in rat brain showed that LPO induced by Fe(2+)/ascorbic acid was dose-dependently reduced by THCQT. According to the biochemical and morphological data, THCQT could protect the liver from CCl(4-)induced injuries.
THCQT seems helpful for protection of liver damage induced by chemicals depending on its anti-oxidant-like function, and THCQT is more effective than silymarin.
阐明中药复方桃核承气汤（THCQT）对四氯化碳（CCl₄）诱导的大鼠肝损伤的保护作用。
将30只雄性Wistar大鼠分为正常对照组、未治疗组、低剂量THCQT组（给予0.3 g/kg THCQT）、高剂量THCQT组（给予0.5 g/kg THCQT）和阳性对照组（给予水飞蓟素25 mg/kg）。所有受试药物在腹腔注射CCl₄（1.5 mL/kg）前1小时口服给药。CCl₄注射24小时后，处死大鼠，观察肝脏组织病理学变化，评估血清天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）活性，以及肝组织中脂质过氧化（LPO）和谷胱甘肽（GSH）水平。
注射CCl₄可升高血清AST和ALT活性，但THCQT可显著逆转这一作用。THCQT可显著降低CCl₄诱导的肝脏LPO增加。此外，该中药合剂还可增加大鼠肝脏GSH含量。组织病理学分析显示，THCQT可减少脂肪堆积、坏死和淋巴细胞浸润。大鼠脑的体外研究表明，THCQT可剂量依赖性降低Fe²⁺/抗坏血酸诱导的LPO。根据生化和形态学数据，THCQT可保护肝脏免受CCl₄诱导的损伤。
THCQT可能因其类似抗氧化的功能有助于保护化学物质诱导的肝损伤，且THCQT比水飞蓟素更有效。
