Department of Biochemistry, University College of Medical Sciences and GTB Hospital, Delhi, India.
Ann Hepatol. 2011 Jul-Sep;10(3):333-9.
The study was designed to evaluate the hepatoprotective activity of aqueous extract of central stem of Musa sapientum (AqMS) against carbon tetrachloride induced hepatotoxicity in rats. Animals were divided into six groups. Group I served as normal control. Group II, III, IV, V & VI were administered CCl(4) mixed with olive oil 1:1 (1.5 mL/kg) I.P., twice a week for 5 weeks. Group II was maintained as CCl(4) intoxicated control. Group III, IV and V received AqMS at a dose of 25, 50 and 100 mg/kg. Group VI received silymarin 100 mg/kg for 5 weeks orally once daily. Marker enzymes of hepatic functions estimated in serum were AST, ALT and ALP. Antioxidant parameters estimated were MDA and GSH in blood and liver and SOD in blood, after fifth week, animals were sacrificed, livers dissected out and evaluated for histomorphological changes.
There was significant rise in AST, ALT and ALP in CCl(4) intoxicated control group II. Treatment with AqMS prevented rise in levels of these enzymes. There was significant rise in MDA and fall in GSH in blood and liver in group II, indicating increased lipid peroxidation and oxidative stress upon CCl(4) administration. Treatment with AqMS prevented rise in MDA & increased GSH in treated group. SOD levels were decreased in group II while groups treated with AqMS showed significant rise (p < 0.05). Maximum hepatoprotective effect was observed with 50 mg/kg dose. Hepatoprotective effect observed with this dose was comparable to standard hepatoprotective drug silymarin. The results of pathological study also support the results of biochemical findings.
the results of the present study indicate that stem of Musa sapientum possess hepatoprotective effect and probably it is due to it's antioxidant property.
本研究旨在评估芭蕉科芭蕉属中央茎水提物（AqMS）对四氯化碳诱导的大鼠肝毒性的保肝活性。动物分为六组。第 I 组为正常对照组。第 II、III、IV、V 和 VI 组每周两次腹腔注射 1:1（1.5 mL/kg）橄榄油混合的 CCl(4)，共 5 周。第 II 组为 CCl(4)中毒对照组。第 III、IV 和 V 组分别给予 AqMS 25、50 和 100 mg/kg。第 VI 组给予水飞蓟素 100 mg/kg，每日 1 次，连续 5 周。第 5 周后，测定血清中肝功能标记酶 AST、ALT 和 ALP。测定血液和肝脏中的 MDA 和 GSH 以及血液中的 SOD 等抗氧化参数。第 5 周后，处死动物，取出肝脏并进行组织形态学评价。
CCl(4)中毒对照组 II 组 AST、ALT 和 ALP 显著升高。用 AqMS 治疗可防止这些酶水平升高。第 II 组血液和肝脏中的 MDA 升高，GSH 降低，表明 CCl(4)给药后脂质过氧化和氧化应激增加。用 AqMS 治疗可防止 MDA 升高和 GSH 降低。第 II 组 SOD 水平降低，而用 AqMS 治疗的各组均显著升高（p<0.05）。50 mg/kg 剂量组的肝保护作用最大。该剂量的肝保护作用与标准肝保护药物水飞蓟素相当。病理研究结果也支持生化研究结果。
本研究结果表明，芭蕉科芭蕉属茎具有保肝作用，可能与其抗氧化特性有关。
