Joseph Joshua Allan, Radhakrishnan Uma, Mutyala Sridhar, Goudar Krishnagouda Shankargouda, Ayyappan Usha Parackal Thachappully, Agarwal Amit
Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India.
Department of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India.
J Nat Sci Biol Med. 2015 Jan-Jun;6(1):183-7. doi: 10.4103/0976-9668.149119.
This study evaluated the antioxidant potential of a polyherbal formulation (Phytocee(™)) in the rodent model.
Four groups of rats (n = 6) were pretreated with Vitamin C (20 mg/kg) or Phytocee(™) (20, 100, and 200 mg/kg), respectively for 10 days. Oxidative stress in rat liver was induced by administration of carbon tetrachloride (CCl4) at 2 ml/kg as a single dose orally to all groups except the vehicle control group. After 24 h of administration of CCl4, hepatic levels of malondialdehyde (MDA), serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), hepatic superoxide dismutase (SOD), catalase, and reduced glutathione (GSH) levels were evaluated.
Phytocee(™) administered groups at all the dose levels significantly reduced the hepatic MDA, serum ALT and AST levels with a marked increase in hepatic SOD and catalase as compared with CCl4 treated group.
The findings suggest that Phytocee(™) markedly reversed the effects of CCl4 induced oxidative stress and can be used as an antioxidant feed supplement.
本研究在啮齿动物模型中评估了一种多草药配方（Phytocee(™)）的抗氧化潜力。
将四组大鼠（每组n = 6）分别用维生素C（20毫克/千克）或Phytocee(™)（20、100和200毫克/千克）预处理10天。除溶剂对照组外，向所有组大鼠单次口服给予2毫升/千克四氯化碳（CCl4）以诱导大鼠肝脏氧化应激。给予CCl4 24小时后，评估肝脏丙二醛（MDA）水平、血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、肝脏超氧化物歧化酶（SOD）、过氧化氢酶和还原型谷胱甘肽（GSH）水平。
与CCl4处理组相比，所有剂量水平的Phytocee(™)给药组均显著降低了肝脏MDA、血清ALT和AST水平，同时肝脏SOD和过氧化氢酶显著增加。
研究结果表明，Phytocee(™)显著逆转了CCl4诱导的氧化应激作用，可作为抗氧化饲料添加剂使用。
