Chemical Industries Research Division, National Research Centre, Elbhooth Street, Dokki, Cairo 12622, Egypt.
Int J Biol Macromol. 2010 Apr 1;46(3):310-6. doi: 10.1016/j.ijbiomac.2010.01.005. Epub 2010 Jan 18.
The combination of gelatin (Gel) with a bioactive component hydroxyapatite (HA) and cartilage powder (CP) to form biocomposites takes advantage of the osteoconductivity and osteoinductivity properties. The studies on bionanocomposites containing HA, CP fillers and Gel are still being conducted. In this present study, the bioactive fillers were loaded onto poly(hydroxylethylmethacrylate) and poly(hydroxylethylmethacrylate-co-methyl methacrylate) grafted gelatin copolymers to produce novel bionanocomposites having osteoconductive and osteoinductive properties. The resulting bionanocomposites were assessed by ATR-IR and SEM-EDX techniques to prove the interaction between different matrices. In vitro behavior of these bionanocomposites was performed in SBF for 21 days at pH 7.4 to verify formation of the apatite layer on the surfaces and its enhancement. The results confirmed the formation of thick plentiful aggregated (hexagonal or spherical) nanoparticles with a bright color (apatite layer) containing carbonate ions onto the surface of composites especially these containing CP and P(HEMA-co-MMA) having bone cement formation in their structure. These novel bionanocomposites have unique bioactivity that can be applied in bone implants as scaffolds and tissue engineering in future.
将明胶（Gel）与生物活性成分羟磷灰石（HA）和软骨粉（CP）结合形成生物复合材料，利用了其骨传导性和骨诱导性。目前仍在对含有 HA、CP 填料和 Gel 的生物纳米复合材料进行研究。在本研究中，将生物活性填料负载到聚（羟乙基甲基丙烯酸酯）和接枝明胶的聚（羟乙基甲基丙烯酸酯-甲基丙烯酸甲酯）共聚物上，以制备具有骨传导性和骨诱导性的新型生物纳米复合材料。通过 ATR-IR 和 SEM-EDX 技术评估所得生物纳米复合材料，以证明不同基质之间的相互作用。将这些生物纳米复合材料在 SBF 中进行体外实验，在 pH 值为 7.4 下培养 21 天，以验证表面上磷灰石层的形成及其增强。结果证实了在复合材料表面形成了厚的丰富的聚集（六方或球形）纳米颗粒，颜色明亮（含有碳酸根离子的磷灰石层），特别是在含有 CP 和 P(HEMA-co-MMA)的复合材料中，其结构具有骨水泥形成。这些新型生物纳米复合材料具有独特的生物活性，可作为支架应用于骨植入物和未来的组织工程。
