Sundaram Jaya, Durance Timothy D, Wang Rizhi
University of British Columbia, Vancouver, BC, Canada.
Acta Biomater. 2008 Jul;4(4):932-42. doi: 10.1016/j.actbio.2008.01.019. Epub 2008 Feb 14.
Hydroxyapatite (HA) is a fundamental mineral-based biomaterial, used for preparing composites for bone repair and regeneration. Gelatin blended with starch results in scaffold composites with enhanced mechanical properties. A gelatin-starch blend reinforced with HA nanocrystals (nHA) gave biocompatible composites with enhanced mechanical properties. In this study, a porous scaffold of gelatin-starch-nHA composites was fabricated through microwave vacuum drying and crosslinking using trisodium citrate. Three different composite scaffolds were prepared at three different percentages of nHA: 20%, 30% and 40%. The microstructures and compositions of the composites were analyzed. Within the porous structure, the nHA crystals were observed to precipitate. The interaction between the gelatin-starch network film and nHA crystalline material was studied using Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction analysis (XRD). XRD reflections showed that there are two different minerals present in the scaffold composite. There were strong reflection peaks close to the 26 degrees and 32 degrees 2theta angles of HA, and close to the 8 degrees and 49 degrees 2theta angles for sodium citrate minerals. The FTIR result suggested that carboxyl groups, C=O and amino groups play crucial roles in HA formation on the surface of a gelatin network.
羟基磷灰石（HA）是一种基本的矿物基生物材料，用于制备骨修复和再生复合材料。明胶与淀粉混合可形成具有增强机械性能的支架复合材料。用HA纳米晶体（nHA）增强的明胶-淀粉共混物可得到具有增强机械性能的生物相容性复合材料。在本研究中，通过微波真空干燥和使用柠檬酸钠交联制备了明胶-淀粉-nHA复合材料的多孔支架。以三种不同百分比的nHA（20%、30%和40%）制备了三种不同的复合支架。对复合材料的微观结构和组成进行了分析。在多孔结构中，观察到nHA晶体沉淀。使用傅里叶变换红外（FTIR）光谱和X射线衍射分析（XRD）研究了明胶-淀粉网络膜与nHA晶体材料之间的相互作用。XRD反射表明支架复合材料中存在两种不同的矿物。在接近HA的26度和32度2θ角处有强反射峰，在接近柠檬酸钠矿物的8度和49度2θ角处也有强反射峰。FTIR结果表明，羧基、C=O和氨基在明胶网络表面HA的形成中起关键作用。
