Oil and Gas Center of Excellence, School of Chemical Engineering, College of Engineering, University of Tehran, PO Box 11155-4563, Tehran, Iran.
Appl Biochem Biotechnol. 2010 Sep;162(2):510-23. doi: 10.1007/s12010-009-8889-0. Epub 2010 Jan 19.
There is a lack of fundamental knowledge about the scale up of biosurfactant production. In order to develop suitable technology of commercialization, carrying out tests in shake flasks and bioreactors was essential. A reactor with integrated foam collector was designed for biosurfactant production using Bacillus subtilis isolated from agricultural soil. The yield of biosurfactant on biomass (Y(p/x)), biosurfactant on sucrose (Y(p/s)), and the volumetric production rate (Y) for shake flask were obtained about 0.45 g g(-1), 0.18 g g(-1), and 0.03 g l(-1) h(-1), respectively. The best condition for bioreactor was 300 rpm and 1.5 vvm, giving Y(x/s), Y(p/x), Y(p/s), and Y of 0.42 g g(-1), 0.595 g g(-1), 0.25 g g(-1), and 0.057 g l(-1) h(-1), respectively. The biosurfactant maximum production, 2.5 g l(-1), was reached in 44 h of growth, which was 28% better than the shake flask. The obtained volumetric oxygen transfer coefficient (K(L)a) values at optimum conditions in the shake flask and the bioreactor were found to be around 0.01 and 0.0117 s(-1), respectively. Comparison of K(L)a values at optimum conditions shows that biosurfactant production scaling up from shake flask to bioreactor can be done with K(L) a as scale up criterion very accurately. Nearly 8% of original oil in place was recovered using this biosurfactant after water flooding in the sand pack.
关于生物表面活性剂生产放大的基础知识还很缺乏。为了开发合适的商业化技术，在摇瓶和生物反应器中进行试验是必不可少的。为了使用从农业土壤中分离出的枯草芽孢杆菌生产生物表面活性剂，设计了一种带有集成泡沫捕集器的反应器。在摇瓶中，生物表面活性剂对生物质（Y(p/x)）、生物表面活性剂对蔗糖（Y(p/s)）和产率（Y）的产量分别约为 0.45 g g(-1)、0.18 g g(-1)和 0.03 g l(-1) h(-1)。生物反应器的最佳条件为 300 rpm 和 1.5 vvm，此时 Y(x/s)、Y(p/x)、Y(p/s)和 Y 的值分别为 0.42 g g(-1)、0.595 g g(-1)、0.25 g g(-1)和 0.057 g l(-1) h(-1)。最大生物表面活性剂产量为 2.5 g l(-1)，在 44 小时的生长时间内达到，比摇瓶提高了 28%。在摇瓶和生物反应器的最佳条件下获得的体积氧传递系数（K(L)a）值分别约为 0.01 和 0.0117 s(-1)。在最佳条件下比较 K(L)a 值表明，从摇瓶到生物反应器的生物表面活性剂生产放大可以非常准确地使用 K(L)a 作为放大标准。在砂包中进行水驱后，使用这种生物表面活性剂回收了近 8%的原始油藏油。
