[The genetic characteristic analysis of fusion gene measles wild viruses of China in 2006].

OBJECTIVE

To study genetic characteristic of fusion gene (F gene) of wild type measles virus circulated in 2006, analysis the variation regular of F gene through comparing with measles virus of 1999-2003.

METHOD

9 representative strains, which isolated from 8 provinces in 2006, were selected. The whole F gene were amplified by reverse transcript-polymerase chain reaction and were sequenced, then they were compared with the nucleotide acid sequences of Chinese measles vaccine strains and the representative strains of 1999-2003, finally the phylogenetic analysis were conducted.

RESULTS

Among 9 representative strains circulated in China in 2006, the homology of nucleotide acid and amino acid were 98.5%-99.8% and 99%-100% respectively, and they were 95.4%-96.2% and 96.7%-97.2% respectively compared with Chinese Vaccine strains, the homology of nucleotide acid and amino acid were 97.9%-99.7% and 98.1%-100% respectively compared with strains of 1999-2003, In F gene, the 3 glycosylation site (aa32, aa64 and aa70), aa112 and aa195, which played the important role to viral fusion, that have no changes.

CONCLUSION

The F genes of measles virus of china in 2006 had no significant variation, the genetic diversity was not predominance comparing with strains of 1999-2003, and important functional sites in Fusion protein didn't change, the measles epidemic was not correlate with the F gene variation. Because F protein play an important role in Measles viruses, it needs to perform the routine monitoring of F gene, which benefit for understanding of epidemic features and genetic variation pattern of wild type Measles viruses.