Xu Song-Too, Wu Hong-Wei, Zhu Zhen
State Key Laboratory for Molecuar Virology and Genetic Engineering, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Preverntion, Beijing 100050, China.
Zhongguo Yi Miao He Mian Yi. 2009 Feb;15(1):1-7.
To study genetic characteristics of fusion gene (F gene) of H1 genotype of wild measles virus circulated in China from 1999 to 2003.
13 H1 genotype representative strains including 8 subgenotype H1a strains and 5 subgenotype H1b strains isolated in mainland China during 1999-2003 were selected. Entire F gene were amplified by reverse transcript-polymerase chain reaction and sequenced, then they were compared with the nucleotide acid sequences of Chinese measles vaccine strains and the representative strains of A, D3, D6, D7 and E genotypes from other countries. The phylogenetic analysis were conducted.
Among 13 representative strains circulated in China during 1999 to 2003, the homology of nucleotide acid and amino acid were 98.1%-99.0% and 98.1%-100% respectively, and were 95.7%-96.2% and 96.9%-97.2% respectively compared with Chinese vaccine strains,while compared with other genotype strains, the homology of nucleotide acid were 94.4%-96.2%. In F gene,the 3 glycosylation site (aa32, aa64 and aa70), aa112 and aa195 which played the important role to viral fusion had no changes.
The F genes of H1 genotype measles virus circulated in China mainland during 1999-2003 had no sig-nificant vibration, and known important functional sites in Fusion protein didn't have deviation, it could be postulated that the structure and functions of F protein of wild Measles viruses circulated in mainland China from 1999 to 2003 were conserved. F protein, however, played an important role in Measles viruses, it need to conducted routine monitoring to F gene. which benefit for understanding of epidemic features and genetic variation pattern of wild Measles viruses.
研究1999年至2003年在中国流行的野生麻疹病毒H1基因型融合基因（F基因）的遗传特征。
选取1999 - 2003年在中国大陆分离的13株H1基因型代表性毒株，其中包括8株H1a亚基因型毒株和5株H1b亚基因型毒株。采用逆转录 - 聚合酶链反应扩增F基因全长并测序，然后与中国麻疹疫苗株以及来自其他国家的A、D3、D6、D7和E基因型代表性毒株的核苷酸序列进行比较，并进行系统发育分析。
1999年至2003年在中国流行的13株代表性毒株中，核苷酸和氨基酸同源性分别为98.1% - 99.0%和98.1% - 100%，与中国疫苗株相比分别为95.7% - 96.2%和96.9% - 97.2%，而与其他基因型毒株相比，核苷酸同源性为94.4% - 96.2%。在F基因中，对病毒融合起重要作用的3个糖基化位点（aa32、aa64和aa70）、aa112和aa195没有变化。
1999 - 2003年在中国大陆流行的H1基因型麻疹病毒F基因无明显变异，融合蛋白中已知的重要功能位点无偏差，可以推测1999年至2003年在中国大陆流行的野生麻疹病毒F蛋白的结构和功能是保守的。然而，F蛋白在麻疹病毒中起重要作用，需要对F基因进行常规监测，这有助于了解野生麻疹病毒的流行特征和遗传变异模式。
