University of Karlsruhe , D-76131 Karlsruhe, Germany.
ACS Nano. 2010 Feb 23;4(2):649-56. doi: 10.1021/nn900886q.
Single-walled carbon nanotubes (SWCNTs) are attractive building blocks for molecular electronics and novel materials. Generating functional architectures with SWCNTs requires methodologies for dispersing, purifying, and binding these highly insoluble quasi one-dimensional molecules. We have previously shown that unstructured DNA strands bind to carbon nanotubes so tightly that it is difficult to address them with complementary strands. Here we show that hairpin oligonucleotides give SWCNT suspensions more concentrated than those obtainable with previously optimized DNA sequences. Further, hairpin-forming oligonucleotides and (6,5)-SWCNTs form complexes that are addressable with complementary, triplex-forming oligonucleotides. As proof of principle, we show that DNA-SWCNT complexes can be bound sequence-specifically with oligonucleotides featuring fluorophores or quantum dots. The new method brings SWCNTs of exquisite purity into the realm of DNA-based nanostructuring.
单壁碳纳米管 (SWCNTs) 是分子电子学和新型材料的有吸引力的构建块。生成具有 SWCNTs 的功能结构需要用于分散、纯化和结合这些高度不溶性准一维分子的方法。我们之前已经表明，无结构的 DNA 链与碳纳米管结合得如此紧密，以至于用互补链来解决它们是很困难的。在这里，我们表明发夹寡核苷酸使 SWCNT 悬浮液比以前优化的 DNA 序列可获得的更浓缩。此外，发夹形成的寡核苷酸和 (6,5)-SWCNTs 形成可与互补的三链形成寡核苷酸寻址的复合物。作为原理证明，我们表明具有荧光团或量子点的寡核苷酸可以特异性地结合 DNA-SWCNT 复合物。该新方法将纯度极高的 SWCNTs 引入基于 DNA 的纳米结构领域。
