Cui Daxiang, Pan Bifeng, Zhang Hong, Gao Feng, Wu Rina, Wang Jingping, He Rong, Asahi Toru
Department of Institute of Micro-Nano Science and Technology, Shanghai JiaoTong University, PR China.
Anal Chem. 2008 Nov 1;80(21):7996-8001. doi: 10.1021/ac800992m. Epub 2008 Sep 25.
A highly selective, ultrasensitive, fluorescence detection method for DNA and antigen based on self-assembly of multiwalled carbon nanotubes (CNTs) and CdSe quantum dots (QDs) via oligonucleotide hybridization is reported. Mercaptoalkyloligonucleotide molecules bind to the quantum dots, while amineoalkyloligonucleotides bind to CNTs with -COCl surface groups. QDs and CNTs further assemble into nanohybrids through DNA hybridization in the presence of target complementary oligonucleotides. The method is achieved with good repeatability with the detection limit of 0.2 pM DNA molecules and 0.01 nM antigen molecules. This novel detection system can also be used for multicomponent detection and antigen-antibody immunoreaction. The novel system has great potential in applications such as ultrasensitive pathogen DNA or antigen or antibody detection, molecular imaging, and photoelectrical biosensors.
报道了一种基于多壁碳纳米管（CNT）和CdSe量子点（QD）通过寡核苷酸杂交自组装的用于DNA和抗原的高选择性、超灵敏荧光检测方法。巯基烷基寡核苷酸分子与量子点结合，而胺基烷基寡核苷酸与具有-COCl表面基团的碳纳米管结合。在存在靶标互补寡核苷酸的情况下，量子点和碳纳米管通过DNA杂交进一步组装成纳米杂化物。该方法具有良好的重复性，DNA分子的检测限为0.2 pM，抗原分子的检测限为0.01 nM。这种新型检测系统还可用于多组分检测和抗原-抗体免疫反应。该新型系统在超灵敏病原体DNA或抗原或抗体检测、分子成像和光电生物传感器等应用中具有巨大潜力。
