Chemical Sciences and Technology Division, National Institute for Interdisciplinary Science and Technology (CSIR), Trivandrum, India.
Int J Food Sci Nutr. 2010 May;61(3):272-81. doi: 10.3109/09637480903379478.
Beta-mannanase was extracted from coconut (Cocos nucifera Linn) haustorium and purified through ammonium sulfate precipitation and sepharose 6B-lectin affinity chromatography. Coconut beta-mannanase is an acidic protein with a pI of 3.75. The molecular mass of coconut beta-mannanase (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) was found to be 44 kDa and was confirmed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The optimum temperature and pH for enzyme activity was 70 degrees C and 5.2. The enzyme was used for the preparation of neutraceutical dietary supplement from galactomannans of guar gum and tender coconut kernel having a beta-(1,4)-linked D-mannose backbone. Depolymerized guar gum has 92% of oligosaccharides with a degree of polymerization of 3 and 7. Tender coconut kernel has a degree of polymerization of 9-39 oligosaccharides along with disaccharides and trisaccharides. Hence this mannanase will be useful to depolymerize beta-(1,4)-linked D-mannose polysaccharides from most plant sources to produce prebiotics in a cost-effective technique.
β-甘露聚糖酶从椰子（Cocos nucifera Linn）吸器中提取，并通过硫酸铵沉淀和琼脂糖 6B-凝集素亲和层析进行纯化。椰子β-甘露聚糖酶是一种酸性蛋白，等电点为 3.75。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳发现椰子β-甘露聚糖酶的分子量为 44 kDa，并通过基质辅助激光解吸电离飞行时间质谱得到证实。酶活性的最适温度和 pH 值分别为 70°C 和 5.2。该酶用于从瓜尔胶的半乳甘露聚糖和嫩椰子仁中制备营养膳食补充剂，其具有β-（1,4）-连接的 D-甘露糖主链。降解的瓜尔胶中有 92%的低聚糖，聚合度为 3 和 7。嫩椰子仁中有 9-39 个低聚糖以及二糖和三糖。因此，这种甘露聚糖酶将有助于从大多数植物来源中降解β-（1,4）-连接的 D-甘露糖多糖，以生产具有成本效益的技术的益生元。
