School of Industrial Technology, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia.
Int J Food Sci Nutr. 2010 Mar;61(2):161-81. doi: 10.3109/09637480903348122.
Lactobacillus sp. FTDC 2113, L. acidophilus FTDC 8033, L. acidophilus ATCC 4356, L. casei ATCC 393, Bifidobacterium FTDC 8943 and B. longum FTDC 8643 were incorporated into soymilk supplemented with fructooligosaccharides (FOS), inulin, mannitol, maltodextrin and pectin. The objective of the present study was to evaluate the effects of prebiotics on the bioactivity of probiotic-fermented soymilk. Proteolytic activity was increased in the presence of FOS, while the supplementation of inulin and pectin increased the angiotensin I-converting enzyme inhibitory activity accompanied by lower IC(50) values. The beta-glucosidase activity was also enhanced in the presence of pectin. This led to higher bioconversion of glucosides to aglycones by probiotics, especially genistin and malonyl genistin to genistein. Results from this study indicated that the supplementation of prebiotics enhanced the in-vitro antihypertensive effect and production of bioactive aglycones in probiotic-fermented soymilk. Therefore, this soymilk could potentially be used as a dietary therapy to reduce the risks of hypertension and hormone-dependent diseases such as breast cancer, prostate cancer and osteoporosis.
植物乳杆菌 FTDC 2113、嗜酸乳杆菌 FTDC 8033、嗜酸乳杆菌 ATCC 4356、干酪乳杆菌 ATCC 393、双歧杆菌 FTDC 8943 和长双歧杆菌 FTDC 8643 被掺入添加了果寡糖 (FOS)、菊粉、甘露醇、麦芽糊精和果胶的豆浆中。本研究的目的是评估益生元对益生菌发酵豆浆生物活性的影响。在 FOS 的存在下，蛋白水解活性增加，而菊粉和果胶的添加增加了血管紧张素 I 转化酶抑制活性，同时伴有较低的 IC(50) 值。果胶的存在也增强了β-葡萄糖苷酶活性。这导致益生菌对糖苷的生物转化更高，特别是染料木苷和丙二酰染料木苷转化为染料木黄酮。本研究结果表明，益生元的添加增强了益生菌发酵豆浆的体外降血压作用和生物活性苷元的产生。因此，这种豆浆可能可作为一种饮食疗法，降低高血压和激素依赖性疾病（如乳腺癌、前列腺癌和骨质疏松症）的风险。
