利用SSR和STS标记对家蚕非鳞翅翅基因nlw进行定位

[Mapping of non-lepis wing gene nlw in silkworm (Bombyx mori) using SSR and STS markers].

作者信息

Wang Xiu-Ye, Li Mu-Wang, Zhao Yun-Po, Xu An-Ying, Guo Qiu-Hong, Huang Yong-Ping, Guo Xi-Jie

机构信息

Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.

出版信息

Yi Chuan. 2010 Jan;32(1):54-8. doi: 10.3724/sp.j.1005.2010.00054.

DOI:10.3724/sp.j.1005.2010.00054

PMID:20085886

Abstract

The non-lepis wing of silkworm (Bombyx mori) is controlled by the recessive gene, nlw. Owning to lack of crossing over in females, the reciprocal backcrossed F(1) (BC(1)) progenies were used for linkage analysis and mapping of nlw based on the SSR linkage map and STS markers using the wild type (+(nlw)/+(nlw)) silkworm strain P50 and U06 with scaleless wing (nlw/nlw). The nlw gene was linked to eight SSR markers and one STS marker. All the individuals with the wild type in the BC1F (Using F(1) as female to backcross to the recessive parent, that is (U06xP50)xU06) showed heterozygous profile of (U06xP50) F(1), and the ones with non-lepis wing in BC1F exhibited the homozygous profile of the strain U06. Using a reciprocal BC1M (Using F1 as male to backcross to the recessive parent, that is U06x(U06xP50))cross, we constructed a linkage map of 125.6 cM, and the distance between nlw and the nearest marker cash2p was 11.4 cM.

摘要

家蚕（Bombyx mori）的无鳞翅由隐性基因nlw控制。由于雌性家蚕缺乏交叉互换，利用野生型（+(nlw)/+(nlw)）家蚕品系P50和无鳞翅（nlw/nlw）的U06品系，通过SSR连锁图谱和STS标记，对回交F(1)（BC(1)）后代进行连锁分析和nlw基因定位。nlw基因与8个SSR标记和1个STS标记连锁。在BC1F（以F(1)为母本回交隐性亲本，即(U06×P50)×U06）中，所有表现为野生型的个体呈现出(U06×P50) F(1)的杂合图谱，而表现为无鳞翅的个体呈现出U06品系的纯合图谱。通过回交BC1M（以F1为父本回交隐性亲本，即U06×(U06×P50)）杂交，构建了一个长度为125.6 cM的连锁图谱，nlw与最近标记cash2p之间的距离为11.4 cM。