利用SSR标记对家蚕（Bombyx mori）中正常标记基因+P进行连锁和定位分析。

Linkage and mapping analyses of the normal marking gene +P in the silkworm (Bombyx mori) using SSR markers.

作者信息

Wei G Q, Yu L, Liu C L, Zhu B J, Ding H J

机构信息

School of Life Science, Anhui Agricultural University, Hefei, Anhui Province, China.

出版信息

Genet Mol Res. 2013 Jul 8;12(3):2351-9. doi: 10.4238/2013.January.22.1.

DOI:10.4238/2013.January.22.1

PMID:23359060

Abstract

In the silkworm, Bombyx mori, normal markings are mainly controlled by the +P gene, which is located on the second chromosome. Due to a lack of crossing over in females, reciprocal backcrossed F1 (BC1) progenies were used for linkage analysis and mapping of the +P gene based on an SSR linkage map using silkworm strains P50 and H9, which are normal marking and sex-limited marking, respectively. The +P gene was found to be linked to 3 SSR markers. Using a reciprocal BC1M cross, we constructed a linkage map of 22.5 cM, with +P mapped at 11.3 cM and the nearest SSR marker S0206 at a distance of 3.0 cM. Based on a fine genome map of domesticated silkworms, Kaikoblast analysis showed that the physical distance between the nearest markers (containing the +P gene) is 995 kb. Further analysis showed that BGIBMGA009689, BGIBMGA009688, and BGIBMGA009687 are closer to +P, and that BGIBMGA009689 is closest to +P, with a physical distance of 19.1 kb.

摘要

在家蚕（Bombyx mori）中，正常斑纹主要由位于第二条染色体上的+P基因控制。由于雌性家蚕缺乏交叉互换，因此利用家蚕品系P50（正常斑纹）和H9（限性斑纹），基于SSR连锁图谱，通过正反交回交F1（BC1）后代进行+P基因的连锁分析和定位。发现+P基因与3个SSR标记连锁。利用正反交BC1M杂交，构建了一个22.5 cM的连锁图谱，+P基因定位于11.3 cM处，最近的SSR标记S0206距离为3.0 cM。基于家蚕精细基因组图谱，Kaikoblast分析表明，最近标记（包含+P基因）之间的物理距离为995 kb。进一步分析表明，BGIBMGA009689、BGIBMGA009688和BGIBMGA009687与+P基因距离更近，其中BGIBMGA009689与+P基因距离最近，物理距离为19.1 kb。