Chen Chi-Yang, Kuo Tsung-Li, Sheu Shi-Yuan, Kuo Tzong-Fu
Graduate Institute of Veterinary Medicine, College of Bio-Resources and Agriculture, National Taiwan University, Taipei.
J Vet Med Sci. 2010 Jun;72(6):679-85. doi: 10.1292/jvms.09-0284. Epub 2010 Jan 20.
It is well known that maintenance therapy using Chai-hu-gui-zhi-tang (CHGZT), a traditional Chinese medicine, has been proven to prevent the recurrence of peptic ulcers. However, little is known as to whether or not it has protective effects against acute gastric injury. In the present study, we investigated the preventive effects of pretreatment with CHGZT extract on the development of water immersion restraint stress-induced acute gastric ulceration in male Wistar rats. The CHGZT extract (50, 250, 500 mg/kg b.w., p.o.) was given to rats before they were exposed to 2 or 4 hr of water immersion restraint stress; they were then were sacrificed immediately after stress exposure. Gastric mucosal lesions were evaluated macroscopically, and the gastric mucosal and hepatic non-protein sulfhydryls (NP-SH) were measured simultaneously. The results indicate that exposure to water immersion restraint stress resulted in the development of acute gastric stress erosions. Pretreatment with CHGZT extract caused a significant reduction of stress lesions and an increase in the gastric mucosal NP-SH and hepatic NP-SH concentrations. We conclude that the anti-ulcer response and extensive antioxidant effect of Chai-hu-gui-zhi-tang may be valuable in prevention of experimental gastric mucosal lesions in rats because it possesses preventive and gastroprotective effects.
众所周知，使用中药柴胡桂枝汤（CHGZT）进行维持治疗已被证明可预防消化性溃疡的复发。然而，关于其对急性胃损伤是否具有保护作用却知之甚少。在本研究中，我们调查了用CHGZT提取物预处理对雄性Wistar大鼠水浸束缚应激诱导的急性胃溃疡形成的预防作用。在大鼠暴露于2或4小时水浸束缚应激之前，给予CHGZT提取物（50、250、500mg/kg体重，口服）；应激暴露后立即将它们处死。宏观评估胃黏膜损伤，并同时测量胃黏膜和肝脏中的非蛋白巯基（NP-SH）。结果表明，暴露于水浸束缚应激会导致急性胃应激性糜烂的形成。用CHGZT提取物预处理可显著减少应激损伤，并增加胃黏膜NP-SH和肝脏NP-SH浓度。我们得出结论，柴胡桂枝汤的抗溃疡反应和广泛的抗氧化作用可能对预防大鼠实验性胃黏膜损伤有价值,因为它具有预防和胃保护作用。
