Li J, Takeda H, Inazu M, Hayashi M, Tsuji M, Ikoshi H, Takada K, Matsumiya T
Department of Pharmacology, Tokyo Medical College, Japan.
Methods Find Exp Clin Pharmacol. 1998 Jan-Feb;20(1):31-7. doi: 10.1358/mf.1998.20.1.485629.
Hange-shashin-to (HST) is a traditional Chinese herbal prescription (Banxia Xiexin Tang) which has long been used in the therapy of gastric functional disorders. In this report, the effect of HST extract on water-immersion restraint stress-induced gastric ulcer based on the changes in gastric mucin content and the variations of monoamine contents in hypothalamus were investigated. Ulcer index was microscopically measured by the sum total of the lengths of ulcers in glandular stomach. Gastric mucin content was determined by a PAS-staining methods and the monoamine contents were detected by HPLC-ECD method. HST extract was orally administered at 1, 2 and 3 g/kg for three consecutive days before stress exposure. Water-immersion restraint stress decreased the mucin content and produced gastric ulcers in a restraint time-dependent manner. Pretreatment with HST extract markedly inhibited the reduction of gastric mucin content and the development of gastric ulcer with significant differences (p < or = 0.01). The remarkable decrease of NE and 5-HT contents but prominent increases of MHPG and 5-HIAA contents were observed in hypothalamus after water-immersion restraint stress (p < or = 0.01). Those changes in monoamine contests in hypothalamus were also significantly inhibited by the pretreatment with HST extract at higher dosage (p < or = 0.05). These results indicate that the changes in gastric mucin content and the variation of monoamines in hypothalamus relate to the pathogenesis of water-immersion restraint stress-induced gastric ulcers, and it is suggested that the prophylactic effects of HST extract on stress induced gastric ulcers may be associated with an increase in gastric mucin content, although the prevention of HST extract on the extraordinary changes in monoamine contents in hypothalamus due to stress cannot be ruled out.
半夏泻心汤（Hange-shashin-to，HST）是一种传统的中药方剂（半夏泻心汤），长期用于治疗胃功能紊乱。在本报告中，基于胃黏液素含量的变化和下丘脑单胺含量的变化，研究了HST提取物对水浸束缚应激诱导的胃溃疡的影响。通过腺胃溃疡长度总和显微镜测量溃疡指数。采用PAS染色法测定胃黏液素含量，采用HPLC-ECD法检测单胺含量。在应激暴露前连续三天口服给予1、2和3 g/kg的HST提取物。水浸束缚应激以束缚时间依赖性方式降低黏液素含量并导致胃溃疡。HST提取物预处理显著抑制胃黏液素含量的降低和胃溃疡的发展，差异有统计学意义（p≤0.01）。水浸束缚应激后下丘脑NE和5-HT含量显著降低，但MHPG和5-HIAA含量显著升高（p≤0.01）。较高剂量的HST提取物预处理也显著抑制了下丘脑单胺含量的这些变化（p≤0.05）。这些结果表明，胃黏液素含量的变化和下丘脑单胺的变化与水浸束缚应激诱导的胃溃疡的发病机制有关，并表明HST提取物对应激诱导的胃溃疡的预防作用可能与胃黏液素含量的增加有关，尽管不能排除HST提取物对下丘脑因应激而导致的单胺含量异常变化的预防作用。
