Department of Neurosurgery, Ajou University School of Medicine, Suwon, Korea.
Neurosurgery. 2010 Feb;66(2):333-42; discussion 342. doi: 10.1227/01.NEU.0000363720.07070.A8.
The objective of this study was to determine the rate and pattern of NSC migration in the brain and its time course after NSC transplantation.
We investigated the tropism of HB1.F3 (F3) immortalized human NSCs in rats bearing U373 human glioma in the brain. Rats received an injection of human U373MG malignant glioma cells into the striatum followed by an injection of F3 cells into the contralateral hemisphere 7 days later. We analyzed the numbers, distribution, and migration rate of NSCs using unbiased stereology.
Approximately 10% of the injected NSCs migrated into the tumor region by 50 minutes after NSC injection. The number of NSCs in the tumor region increased slowly up to 5 days post-injection and increased significantly up to 15 days post-injection. Changes in tumor volume showed similar patterns. The rate of NSC migration was approximately 175 microm/min. NSCs increased in number approximately 1.7-fold during day 1 in the absence of tumor cell inoculation in vivo. However, the proliferation of NSCs began to decline after 5 days after injection.
We identified for the first time the rate and pattern of NSC migration to the tumor mass in vivo. These findings may provide useful information with respect to preclinical research of gene therapy for malignant gliomas.
本研究旨在确定神经干细胞（NSC）在脑内的迁移率和模式及其移植后的时间进程。
我们研究了 HB1.F3（F3）永生化人神经干细胞在脑内携带 U373 人胶质细胞瘤大鼠中的趋向性。大鼠在纹状体中接受人 U373MG 恶性神经胶质瘤细胞注射，7 天后对侧半球注射 F3 细胞。我们使用无偏立体学分析来分析 NSCs 的数量、分布和迁移率。
大约 10%的注射 NSCs 在 NSC 注射后 50 分钟内迁移到肿瘤区域。在注射后 5 天内，肿瘤区域的 NSCs 数量缓慢增加，直到 15 天。肿瘤体积的变化呈现出相似的模式。NSC 的迁移率约为 175μm/min。在体内没有肿瘤细胞接种的情况下，NSCs 在第 1 天的数量增加了大约 1.7 倍。然而，在注射后 5 天后，NSCs 的增殖开始下降。
我们首次确定了 NSC 向肿瘤块体内迁移的速度和模式。这些发现可能为恶性神经胶质瘤基因治疗的临床前研究提供有用的信息。
