希腊优秀田径运动员的ACE I/D基因多态性
The ACE I/D polymorphism in elite Greek track and field athletes.
作者信息
Papadimitriou I D, Papadopoulos C, Kouvatsi A, Triantaphyllidis C
机构信息
Laboratory of Sports Biomechanics, Aristotle University, Serres, Greece.
出版信息
J Sports Med Phys Fitness. 2009 Dec;49(4):459-63.
AIM
The aim of this study was to examine genetic differences among 101 elite Greek track and field athletes and a representative random control group of 181 Greek individuals, by analyzing the I/D polymorphism in exon 16 of the ACE gene.
METHODS
Athletes were defined as elite and included in the sample if they had been chosen to represent Greece at the international level. Amplification of DNA was carried out by polymerase chain reaction (PCR). The protein C reactive (PCR) products were separated by electrophoresis on agarose gel and were visualized by UV light. To avoid misclassification of ID genotypes, a second PCR was performed using specific primers.
RESULTS
The ACE genotype and allele frequencies in the top power and endurance oriented athletes were not statistically significant different from those in a representative random sample of the Greek population. There was found only a trend towards an increased in frequency of the ACE DD genotype in the sprinters group (55.88% vs. 31.49%).
CONCLUSIONS
The results suggest weak evidence that the ACE DD genotype could influence sprint performance in Greek athletes.
目的
本研究旨在通过分析血管紧张素转换酶（ACE）基因第16外显子的I/D多态性，检测101名希腊优秀田径运动员与181名希腊个体组成的代表性随机对照组之间的基因差异。
方法
若运动员被选来代表希腊参加国际比赛，则被定义为优秀运动员并纳入样本。通过聚合酶链反应（PCR）进行DNA扩增。蛋白C反应（PCR）产物在琼脂糖凝胶上进行电泳分离，并用紫外线观察。为避免ID基因型的错误分类，使用特异性引物进行第二次PCR。
结果
顶级力量型和耐力型运动员的ACE基因型和等位基因频率与希腊人群代表性随机样本相比，无统计学显著差异。仅在短跑运动员组中发现ACE DD基因型频率有增加趋势（55.88%对31.49%）。
结论
结果表明，有微弱证据显示ACE DD基因型可能影响希腊运动员的短跑成绩。
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