ACTN3（R/X）和 ACE（I/D）基因多态性及其训练后表达在 CrossFit 运动员中的研究。
Prevalence of Polymorphism and Post-Training Expression of ACTN3 (R/X) and ACE (I/D) Genes in CrossFit Athletes.
机构信息
Faculty of Physical Culture Sciences, Autonomous University of Chihuahua, Campus II, Circuito Universitario S/N, Chihuahua 31125, Mexico.
PABIOM Laboratory, Faculty of Medicine and Biomedical Sciences, Autonomous University of Chihuahua, Campus II, Circuito Universitario S/N, Chihuahua 31109, Mexico.
出版信息
Int J Environ Res Public Health. 2023 Mar 1;20(5):4404. doi: 10.3390/ijerph20054404.
BACKGROUND
CrossFit is known as a functional fitness training high-intensity exercise to improve physical performance. The most studied polymorphisms are the ACTN3 R577X gene, known for speed, power, and strength, and ACE I/D, related to endurance and strength. The present investigation analyzed the effects of training on ACTN3 and ACE gene expression in CrossFit athletes for 12 weeks.
METHODS
the studies included 18 athletes from the Rx category, where ACTN3 (RR, RX, XX) and ACE (II, ID, DD) characterization of genotypes and tests of maximum strength (NSCA), power (T-Force), and aerobic endurance (Course Navette) were performed. The technique used was the reverse transcription-quantitative PCR real-time polymerase chain reaction (RT-qPCR) for the relative expression analysis.
RESULTS
the relative quantification (RQ) values for the ACTN3 gene increased their levels 2.3 times ( = 0.035), and for ACE, they increased 3.0 times ( = 0.049).
CONCLUSIONS
there is an overexpression of the ACTN3 and ACE genes due to the effect of training for 12 weeks. Additionally, the correlation of the expression of the ACTN3 ( = 0.040) and ACE ( = 0.030) genes with power was verified.
背景
CrossFit 被称为一种功能性健身训练的高强度运动，可提高身体表现。研究最多的多态性是 ACTN3 R577X 基因，该基因与速度、力量和强度有关，而 ACE I/D 则与耐力和力量有关。本研究分析了 12 周 CrossFit 运动员训练对 ACTN3 和 ACE 基因表达的影响。
方法
该研究纳入了 18 名 Rx 类别的运动员，对其 ACTN3（RR、RX、XX）和 ACE（II、ID、DD）基因型特征和最大力量（NSCA）、力量（T-Force）和有氧耐力（Course Navette）测试进行了测试。使用的技术是逆转录定量聚合酶链反应实时聚合酶链反应（RT-qPCR）进行相对表达分析。
结果
ACTN3 基因的相对定量（RQ）值增加了 2.3 倍（ = 0.035），ACE 基因增加了 3.0 倍（ = 0.049）。
结论
经过 12 周的训练，ACTN3 和 ACE 基因的表达过度。此外，还验证了 ACTN3（ = 0.040）和 ACE（ = 0.030）基因与力量的表达之间存在相关性。
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