Liu Qiyong, Sun Jimin, Lu Liang, Fu Guiming, Ding Gangqiang, Song Xiuping, Meng Fengxia, Wu Haixia, Yang Tianci, Ren Zhangyao, Chen Enfu, Lin Junfen, Lv Huakun, Chai Chengliang
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No 155, Changping District, Beijing 102206, China.
J Wildl Dis. 2010 Jan;46(1):179-85. doi: 10.7589/0090-3558-46.1.179.
To estimate the prevalence of Bartonella in small mammals of different species, during different seasons, and at different study sites, and to provide baseline data for the risk assessment of human Bartonella infection, we captured small mammals using snap traps in Zhejiang Province, China. Bartonella species were detected in small-mammal samples by polymerase chain reaction and positive amplicons were sequenced. Bartonella DNA was detected in 47% (90/192) of Apodemus agrarius, 31% (14/45) of Rattus losea, 16% (7/43) of Rattus norvegicus, 24% (9/37) of Eothenomys melanogaster, 4% (1/28) of Niviventer confucianus, 30% (7/23) of Suncus murinus, 22% (2/9) of Microtus fortis, 27% (2/7) of Rattus tanezumi, and 29% (2/7) of Apodemus peninsulae. No Bartonella DNA was detected in 27 unidentified Soricidae or nine Mus musculus. This is the first report of Bartonella DNA detected in E. melanogaster and N. confucianus. The prevalence of Bartonella DNA varied among small-mammal species, study sites, and seasons; the prevalence of Bartonella DNA between genders did not vary significantly within a species. The sequences we report were most similar to Bartonella grahamii.
为了估计不同物种、不同季节和不同研究地点的小型哺乳动物中巴尔通体的流行情况,并为人类巴尔通体感染的风险评估提供基线数据,我们在中国浙江省使用 snap 陷阱捕获小型哺乳动物。通过聚合酶链反应在小型哺乳动物样本中检测巴尔通体物种,并对阳性扩增子进行测序。在黑线姬鼠的 47%(90/192)、黄毛鼠的 31%(14/45)、褐家鼠的 16%(7/43)、黑腹绒鼠的 24%(9/37)、针毛鼠的 4%(1/28)、臭鼩鼱的 30%(7/23)、东方田鼠的 22%(2/9)、黄胸鼠的 27%(2/7)和半岛姬鼠的 29%(2/7)中检测到巴尔通体 DNA。在 27 只未鉴定的鼩鼱科动物或 9 只小家鼠中未检测到巴尔通体 DNA。这是首次在黑腹绒鼠和针毛鼠中检测到巴尔通体 DNA 的报告。巴尔通体 DNA 的流行率在小型哺乳动物物种、研究地点和季节之间有所不同;在一个物种内,巴尔通体 DNA 的流行率在性别之间没有显著差异。我们报告的序列与格雷厄姆巴尔通体最相似。