Department of Surgery, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, OH 45267, USA.
Shock. 2010 Jul;34(1):40-5. doi: 10.1097/SHK.0b013e3181d494fd.
Blood transfusion remains an essential treatment of acute anemia. Current storage processes allow the efficient administration of blood products. Erythrocytes undergo morphological and biochemical changes during storage that may affect outcomes after transfusion. A reliable small-animal model would be ideal to examine the effects of stored blood products after transfusion. The objective of this study was to characterize the storage of murine erythrocytes for future application to animal models of acute anemia. Blood samples were collected from male mice and human volunteers, separated into components, and stored. At intervals, morphological and biochemical analysis was performed. Lactate, potassium, hemoglobin, and hemolysis were determined, and cell morphology was evaluated with light microscopy. Murine packed red blood cells (pRBCs) aged more rapidly than human samples. Murine pRBCs exhibited higher lactate levels (34.9 +/- 1.3 mmol/L vs. 18.1 +/- 1.0 mmol/L, mouse vs. human) and more severe acidosis as indicated by pH (6.56 +/- 0.02 vs. 6.79 +/- 0.04, mouse vs. human). Murine pRBCs hemolyzed earlier (11.2 +/- 3.7 g vs. 0.7 +/- 0.3 g, mouse vs. human after 21 days of storage) and more rapidly than human pRBCs. Corpuscular changes consistent with red cell storage lesions appeared earlier in murine samples compared with human stored pRBCs. Compared with human pRBCs, murine pRBCs exhibit similar but more accelerated aging processes under standard storage conditions. Characterization of the murine red cell storage lesion will allow the application of stored blood components to future investigations into the treatment of acute anemia in experimental murine models.
输血仍然是急性贫血的基本治疗方法。目前的储存过程允许有效地管理血液制品。红细胞在储存过程中会发生形态和生化变化，这可能会影响输血后的结果。一个可靠的小动物模型将是研究输血后储存血液制品影响的理想模型。本研究的目的是描述鼠红细胞的储存，以便将来应用于急性贫血的动物模型。从雄性小鼠和人类志愿者中采集血液样本，分离成成分并储存。每隔一段时间进行形态和生化分析。测定乳酸、钾、血红蛋白和溶血情况，并通过光镜评估细胞形态。鼠浓缩红细胞（pRBC）比人样本衰老得更快。鼠 pRBC 的乳酸水平更高（34.9 +/- 1.3 mmol/L 比 18.1 +/- 1.0 mmol/L，鼠比人），酸度更严重，pH 值更低（6.56 +/- 0.02 比 6.79 +/- 0.04，鼠比人）。鼠 pRBC 更早（21 天后 11.2 +/- 3.7 g 比 0.7 +/- 0.3 g，鼠比人）和更快地发生溶血。与人类储存的 pRBC 相比，鼠样本中出现了与红细胞储存损伤一致的胞体变化，这一变化出现得更早。与人类 pRBC 相比，鼠 pRBC 在标准储存条件下表现出相似但更加速的衰老过程。鼠红细胞储存损伤的特征将允许将储存的血液成分应用于未来对实验性鼠模型急性贫血治疗的研究。
