Johnson L D, Lazzarini R A
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4387-91. doi: 10.1073/pnas.74.10.4387.
The genome of a defective interfering particle (DILT) derived from the heat-resistant strain of vesicular stomatitis virus is expressed in vivo without the assistance of infectious helper virus. The rates of RNA synthesis in the presence of cycloheximide (primary transcription) are the same when infections are with equal numbers of physical particles of DILT or virus. With this treatment, DILT synthesizes only 12-17S mRNAs as characterized by size, polarity, and polyadenylylation. In the absence of cycloheximide, DILT-infected cells produce not only these mRNAs but also a 28S RNA species. This RNA, which represents one half of the viral specific RNA, contains newly synthesized full-length (+) and (-) strand DILT RNA. Both strands are found intracellularly as ribonucleoprotein complexes. Without cycloheximide present, the rate of RNA synthesis by DILT was less than that by virus. This curtailment is most likely due to the inability of DILT to synthesize L protein mRNA. An expanded role for defective interfering particles in infection is discussed.
源自水疱性口炎病毒耐热株的缺陷干扰颗粒（DILT）的基因组在没有感染性辅助病毒的情况下在体内表达。当用等量的DILT或病毒的物理颗粒进行感染时，在存在环己酰亚胺的情况下（初级转录）RNA合成速率相同。经过这种处理，DILT仅合成大小、极性和多聚腺苷酸化特征为12 - 17S的mRNA。在没有环己酰亚胺的情况下，感染DILT的细胞不仅产生这些mRNA，还产生一种28S RNA。这种RNA占病毒特异性RNA的一半，包含新合成的全长（+）和（ - ）链DILT RNA。两条链都作为核糖核蛋白复合物存在于细胞内。在没有环己酰亚胺的情况下，DILT的RNA合成速率低于病毒。这种减少很可能是由于DILT无法合成L蛋白mRNA。文中讨论了缺陷干扰颗粒在感染中的扩展作用。
