Chanda P K, Kang C Y, Banerjee A K
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3927-31. doi: 10.1073/pnas.77.7.3927.
Under appropriate reaction conditions in vitro, four different defective-interfering particles of vesicular stomatitis virus have been shown to synthesize the full-length complement of their RNAs. The reaction involved preinitiation of the core particles with ATP and CTP, followed by RNA chain elongation in the presence of the beta, gamma-imido analogue of ATP, AdoPP[NH]P, and the three normal ribonucleoside triphosphates. By hybridization of the in vitro synthesized plus strand with the standard genome RNA followed by RNase treatment of the heteroduplexes, we have shown that the RNA of a defective-interfering particle derived from the 3' end of the genome RNA has evolved by an internal deletion of the standard genome.
在体外适当的反应条件下，已证明水疱性口炎病毒的四种不同缺陷干扰颗粒能够合成其RNA的全长互补序列。该反应包括用ATP和CTP对核心颗粒进行预起始，随后在ATP的β,γ-亚氨基类似物AdoPP[NH]P以及三种正常核糖核苷三磷酸存在的情况下进行RNA链延伸。通过将体外合成的正链与标准基因组RNA杂交，然后对异源双链体进行核糖核酸酶处理，我们已经表明，源自基因组RNA 3'端的缺陷干扰颗粒的RNA是通过标准基因组的内部缺失而进化的。
