School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China.
J Biomed Mater Res B Appl Biomater. 2010 Apr;93(1):84-92. doi: 10.1002/jbm.b.31561.
The objective of this study was to synthesize and characterize novel three-dimensional porous scaffolds made of poly(lactic-co-glycolic acid) (PLGA)/nano-TiO(2)-particle composite microspheres for potential bone repair applications. The introduction of TiO(2) component has been proven capable of largely enhancing mechanical properties of PLGA/TiO(2) microsphere-sintered scaffold ("PLGA/TiO(2)-SMS"). In addition, composite nano-TiO(2) additives are capable of inducing an increased arrest of adhesive proteins from the environment, which benefits cell attachment onto the scaffolds. Osteoblast proliferation and maturation were evaluated by MTT assay, alkaline phosphatase (ALP) activity, and bony calcification assay. The results indicate that osteoblasts cultured on the composite scaffolds with different TiO(2) content (0, 0.1, and 0.3 g/1 g PLGA) display increased cell proliferation compared with pure PLGA scaffold. When cultured on composite scaffolds, osteoblasts also exhibit significantly enhanced ALP activity and higher calcium secretion, with respect to those on the pure PLGA scaffolds. Taken together, PLGA/TiO(2)-SMSs deserve attention utilizing for potential bone-repairing therapeutics.
本研究的目的是合成和表征新型三维多孔支架，由聚（乳酸-共-乙醇酸）（PLGA）/纳米 TiO2-颗粒复合材料微球组成，用于潜在的骨修复应用。已经证明 TiO2成分的引入能够极大地提高 PLGA/TiO2微球烧结支架（“PLGA/TiO2-SMS”）的机械性能。此外，复合纳米 TiO2添加剂能够诱导环境中附着蛋白的附着增加，有利于细胞附着在支架上。通过 MTT 测定、碱性磷酸酶（ALP）活性和骨钙化测定来评估成骨细胞的增殖和成熟。结果表明，与纯 PLGA 支架相比，在不同 TiO2含量（0、0.1 和 0.3 g/1 g PLGA）的复合支架上培养的成骨细胞显示出更高的细胞增殖。与纯 PLGA 支架相比，在复合支架上培养的成骨细胞的 ALP 活性和钙分泌也显著增强。综上所述，PLGA/TiO2-SMS 值得关注，可用于潜在的骨修复治疗。
