Biomaterial Group, Faculty of Biomedical Engineering, Amirkabir University of Technology, P.O. Box: 15875-4413, Tehran, Iran.
Appl Biochem Biotechnol. 2014 Mar;172(5):2465-79. doi: 10.1007/s12010-013-0696-y. Epub 2014 Jan 7.
In this research, the novel three-dimensional (3D) porous scaffolds made of poly(lactic-co-glycolic acid) (PLGA)/nano-fluorohydroxyapatite (FHA) composite microspheres was prepared and characterize for potential bone repair applications. We employed a microsphere sintering method to produce 3D PLGA/nano-FHA scaffolds composite microspheres. The mechanical properties, pore size, and porosity of the composite scaffolds were controlled by varying parameters, such as sintering temperature, sintering time, and PLGA/nano-FHA ratio. The experimental results showed that the PLGA/nano-FHA (4:1) scaffold sintered at 90 °C for 2 h demonstrated the highest mechanical properties and an appropriate pore structure for bone tissue engineering applications. Furthermore, MTT assay and alkaline phosphatase activity (ALP activity) results ascertained that a general trend of increasing in cell viability was seen for PLGA/nano-FHA (4:1) scaffold sintered at 90 °C for 2 h by time with compared to control group. Eventually, obtained experimental results demonstrated PLGA/nano-FHA microsphere-sintered scaffold deserve attention utilizing for bone tissue engineering.
在这项研究中，我们制备了一种新型的三维（3D）多孔支架，由聚乳酸-共-羟基乙酸（PLGA）/纳米氟羟基磷灰石（FHA）复合微球组成，用于潜在的骨修复应用。我们采用微球烧结法制备 3D PLGA/nano-FHA 支架复合微球。通过改变烧结温度、烧结时间和 PLGA/nano-FHA 比例等参数，可以控制复合支架的力学性能、孔径和孔隙率。实验结果表明，在 90°C 下烧结 2 小时的 PLGA/nano-FHA（4:1）支架具有最高的力学性能和适合骨组织工程应用的孔结构。此外，MTT 测定和碱性磷酸酶活性（ALP 活性）结果表明，与对照组相比，PLGA/nano-FHA（4:1）支架在 90°C 下烧结 2 小时的细胞活力呈逐渐增加的趋势。最终，实验结果表明，PLGA/nano-FHA 微球烧结支架值得关注，可用于骨组织工程。
