Holownia P, Owen E J, Hampl R, Jacobs H S, Honour J W
Department of Chemical Pathology, University College and Middlesex School of Medicine, London, England.
J Steroid Biochem Mol Biol. 1991 Mar;38(3):389-98. doi: 10.1016/0960-0760(91)90112-i.
The development of a chromatographic/immunoassay method is presented for the measurement of 11 beta-hydroxyandrostenedione (11 beta-OH-A4) in ovarian follicular fluid (FFL) and plasma from women undergoing embryo transfer for in vitro fertilization. This method incorporates high-performance liquid chromatography (HPLC) and permits the simultaneous measurement of other steroids from a single sample in order to assess the intraovarian environment. Authenticity of 11 beta-OH-A4 in follicular fluid was confirmed using selected ion monitoring (SIM) gas chromatography/mass spectrometry (GC/MS). Our results demonstrate a mean concentration of 18.6 nmol/l in follicular fluid compared with 3.2 nmol/l in plasma. The origin of 11 beta-OH-A4 in follicular fluid requires further investigation but these findings supports the hypothesis of ovarian 11 beta-hydroxylase activity on C19 steroids.
本文介绍了一种色谱/免疫分析方法的开发，用于测量接受体外受精胚胎移植的女性的卵泡液（FFL）和血浆中的11β-羟基雄烯二酮（11β-OH-A4）。该方法采用高效液相色谱（HPLC），并允许从单个样品中同时测量其他类固醇，以评估卵巢内环境。使用选择离子监测（SIM）气相色谱/质谱（GC/MS）确认了卵泡液中11β-OH-A4的真实性。我们的结果表明，卵泡液中的平均浓度为18.6 nmol/l，而血浆中的平均浓度为3.2 nmol/l。卵泡液中11β-OH-A4的来源需要进一步研究，但这些发现支持了卵巢对C19类固醇具有11β-羟化酶活性的假设。
