Khalil M W, Walton J S
J Endocrinol. 1985 Dec;107(3):375-81. doi: 10.1677/joe.0.1070375.
4-Oestren-3,17-dione (19-norandrostenedione) has been identified as a major steroid in porcine ovarian follicular fluid for the first time. This steroid was isolated by C18 reverse-phase high performance liquid chromatography (HPLC) and its structure was established unambiguously by capillary gas chromatography-mass spectrometry. The mean concentration in pooled fluid obtained from prepubertal gilts determined by HPLC and capillary gas chromatography was 1.71 mumol/l. The leve of this steroid in fluid obtained from large preovulatory follicles of sows exceeded 21.0 mumol/l on days 19 and 20 of the oestrous cycle. The high levels of this steroid within the follicle during the follicular phase might imply a possible biological role in follicular development and oocyte maturation.
4-雌甾烯-3,17-二酮（19-去甲雄烯二酮）首次被鉴定为猪卵巢卵泡液中的一种主要甾体。该甾体通过C18反相高效液相色谱法（HPLC）分离，并通过毛细管气相色谱-质谱法明确确定其结构。通过HPLC和毛细管气相色谱法测定，从青春期前后备母猪获得的混合卵泡液中的平均浓度为1.71μmol/L。在发情周期的第19天和第20天，从母猪大的排卵前卵泡获得的卵泡液中该甾体的水平超过21.0μmol/L。卵泡期卵泡内该甾体的高水平可能意味着其在卵泡发育和卵母细胞成熟中可能具有生物学作用。
