Food and Nutritional Sciences Programme, Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China.
J Nutr Biochem. 2010 Nov;21(11):1134-9. doi: 10.1016/j.jnutbio.2009.10.007. Epub 2010 Jan 25.
Interest in grape seed proanthocyanidin (GSP) as a cholesterol-lowering nutraceutical is growing. This study was to investigate the effect of GSP on blood cholesterol level and gene expression of cholesterol-regulating enzymes in Golden Syrian hamsters maintained on a 0.1% cholesterol diet. Results affirmed supplementation of 0.5% or 1.0% GSP could decrease plasma total cholesterol and triacylglycerol level. Western blot and real-time polymerase chain reaction analyses demonstrated GSP did not affect sterol regulatory element binding protein-2 and low-density lipoprotein receptor; however, it increased mRNA 3-hydroxy-3-methylglutaryl coenzyme A reductase. GSP had no effect on the protein mass of liver X receptor alpha (LXRα) but it decreased mRNA LXRα. Most importantly, GSP increased not only the protein level of cholesterol-7α-hydroxylase (CYP7A1) but also mRNA CYP7A1. It was concluded that the hypocholesterolemic activity of GSP was most likely mediated by enhancement of bile acid excretion and up-regulation of CYP7A1.
人们对葡萄籽原花青素（GSP）作为一种降低胆固醇的营养保健品的兴趣与日俱增。本研究旨在探讨 GSP 对给予 0.1%胆固醇饮食的金黄地鼠的血液胆固醇水平和胆固醇调节酶基因表达的影响。结果证实，补充 0.5%或 1.0%的 GSP 可降低血浆总胆固醇和三酰甘油水平。Western blot 和实时聚合酶链反应分析表明，GSP 对固醇调节元件结合蛋白-2 和低密度脂蛋白受体没有影响；然而，它增加了 3-羟-3-甲基戊二酰辅酶 A 还原酶的 mRNA。GSP 对肝 X 受体α（LXRα）的蛋白质量没有影响，但降低了 LXRα的 mRNA。最重要的是，GSP 不仅增加了胆固醇 7α-羟化酶（CYP7A1）的蛋白水平，还增加了 CYP7A1 的 mRNA。综上所述，GSP 的降胆固醇活性可能是通过增强胆汁酸排泄和上调 CYP7A1 来介导的。
