Cold Spring Harbor Laboratory, NY 11724, USA.
Brief Funct Genomics. 2010 Mar;9(2):129-38. doi: 10.1093/bfgp/elp060. Epub 2010 Jan 21.
First discovered in jellyfish, fluorescent proteins (FPs) have been successfully optimized for use as effective biomarkers within living plant cells. When exposed to light, FPs fused to a protein or regulatory element will fluoresce, and non-invasively mark expression and protein localization, which allows for the in vivo monitoring of diverse cellular processes. In this review, we discuss how FP technology has evolved from small-scale analysis of individual genes to more high-throughput techniques for global expression and functional profiling in plants.
最初在水母中发现的荧光蛋白（FPs）已成功优化，可作为活植物细胞内有效生物标志物使用。当暴露在光线下时，与蛋白质或调节元件融合的 FPs 将发出荧光，并无创地标记表达和蛋白质定位，从而能够在体内监测各种细胞过程。在这篇综述中，我们讨论了 FP 技术如何从对单个基因的小规模分析演变为更适用于植物中全局表达和功能分析的高通量技术。
