Boyce Thompson Institute, Cornell University, Ithaca, NY 14853, USA.
Brief Funct Genomics. 2010 Mar;9(2):118-28. doi: 10.1093/bfgp/elp057. Epub 2010 Feb 3.
Ultra high-throughput sequencing (UHTS) technologies offer the potential to interrogate transcriptomes in detail that has traditionally been restricted to single gene surveys. For instance, it is now possible to globally define transcription start sites, polyadenylation signals, alternative splice sites and generate quantitative data on gene transcript accumulation in single tissues or cell types. These technologies are thus paving the way for whole genome transcriptomics and will undoubtedly lead to novel insights into plant development and biotic and abiotic stress responses. However, several challenges exist to making this technology broadly accessible to the plant research community. These include the current need for a computationally intensive analysis of data sets, a lack of standardized alignment and formatting procedures and a relatively small number of analytical software packages to interpret UHTS outputs. In this review we summarize recent findings from UHTS and discuss potential opportunities and challenges for broad adoption of these technologies in the plant science community.
超高通量测序 (UHTS) 技术提供了详细研究转录组的潜力，而传统上这一直局限于单个基因调查。例如，现在可以全局定义转录起始位点、多聚腺苷酸化信号、选择性剪接位点，并在单个组织或细胞类型中生成关于基因转录积累的定量数据。这些技术为全基因组转录组学铺平了道路，无疑将为植物发育以及生物和非生物胁迫反应带来新的见解。然而，要使这项技术广泛应用于植物研究界，还存在一些挑战。这些挑战包括当前对数据集进行计算密集型分析的需求、缺乏标准化的对齐和格式化程序以及相对较少的分析软件包来解释 UHTS 输出。在这篇综述中，我们总结了 UHTS 的最新发现，并讨论了在植物科学界广泛采用这些技术的潜在机会和挑战。
