Gonzalez S I, Chandrashekar V, Shire J G, Lüthy I A, Bartke A, Calandra R S
Department of Physiology, School of Medicine, Southern Illinois University, Carbondale 62901.
Biol Reprod. 1991 Feb;44(2):321-6. doi: 10.1095/biolreprod44.2.321.
Prolactin (PRL) has been shown to exert many different actions in various biological systems. Polyamines are known to influence the growth and function of the seminal vesicles (SV). Furthermore, ornithine decarboxylase (ODC) is considered a key enzyme in the biosynthesis of polyamines and is regulated by PRL in certain target tissues. Adult Ames dwarf mice (df/df), genetically deficient in PRL, were used for this study. The experimental groups were as follows: Group 1, pituitary-grafted; Group 2, sham-operated; Group 3, castrated + testosterone propionate (TP)-treated (25 micrograms/mouse, 3 times/wk, s.c.) + grafted; and Group 4, castrated + TP as above. The animals were killed 40 days later, and polyamines and ODC activity in SV and liver were determined. Serum PRL, FSH, and testosterone (T) were also measured. In the grafted groups, there were significant elevations in serum PRL and FSH levels. In the gonad-intact, pituitary-grafted group, animals exhibited an elevation in plasma T levels, and similar levels were achieved in the castrated, androgen-replaced groups. In hyperprolactinemic mice, the weights of SV were significantly greater than in the corresponding control groups. The relative weights of the SV showed a similar pattern. An increase in ODC activity was observed in both SV and liver in hyperprolactinemic groups. In those animals in which serum T levels were held constant, an increase in the enzyme activity in SV was detected in hyperprolactinemic group whereas in liver, no significant difference was observed. Concentrations of polyamines in the SV were increased in hyperprolactinemic, castrated, TP-treated mice. The present results indicate that PRL can exert a direct stimulatory effect on the growth, ODC activity, and polyamine levels in the SV.
催乳素（PRL）已被证明在各种生物系统中发挥多种不同作用。多胺已知会影响精囊（SV）的生长和功能。此外，鸟氨酸脱羧酶（ODC）被认为是多胺生物合成中的关键酶，并且在某些靶组织中受PRL调节。本研究使用了成年的艾姆斯侏儒小鼠（df/df），其基因缺陷导致PRL缺乏。实验组如下：第1组，垂体移植组；第2组，假手术组；第3组，去势 + 丙酸睾酮（TP）处理（25微克/只小鼠，每周3次，皮下注射） + 移植组；第4组，去势 + 上述TP处理组。40天后处死动物，测定精囊和肝脏中的多胺及ODC活性。还测量了血清PRL、促卵泡激素（FSH）和睾酮（T）。在移植组中，血清PRL和FSH水平显著升高。在性腺完整的垂体移植组中，动物血浆T水平升高，去势并雄激素替代的组也达到了相似水平。在高催乳素血症小鼠中，精囊重量显著大于相应对照组。精囊的相对重量呈现相似模式。高催乳素血症组的精囊和肝脏中ODC活性均增加。在血清T水平保持恒定的动物中，高催乳素血症组精囊中的酶活性增加，而在肝脏中未观察到显著差异。高催乳素血症、去势、TP处理的小鼠精囊中多胺浓度增加。目前的结果表明，PRL可对精囊的生长、ODC活性和多胺水平发挥直接刺激作用。
