de Queiroz Genilson Fernandes, Dagli Maria Lucia Zaidan, Fukumasu Heidge, Zavala Arturo Alejandro Zavala, Matera Julia Maria
Av. Prof. Orlando M. de Paiva, 87, Departamento de Clínica Cirúrgica, Cidade Universitária, São Paulo, SP, CEP: 05508-000.
J Vet Diagn Invest. 2010 Jan;22(1):105-8. doi: 10.1177/104063871002200121.
The aim of the current study was to evaluate the expression of vascular endothelial growth factor (VEGF) and the microvascular density in canine soft-tissue sarcomas. Immunohistochemistry for VEGF expression was performed on 20 canine neoplasms by the streptavidin-biotin-peroxidase method using an anti-VEGF mouse monoclonal antibody (ab-119). The volume fraction of microvessels in the sarcomas was quantified in hematoxylin and eosin-stained tissue sections. At least 10 fields of view (40x magnification) per neoplasm were analyzed by positioning a grid with 100 points and counting the microvessels that fell into the intersection points. This percentage was considered the volume fraction of these microvessels in the tumor section. VEGF expression was detected in 65% of the neoplasms. In 92.3% of the neoplasms, the expression occurred in the peritumor region; in 46.15%, in the intratumor region; and in 38.46%, the expression was present in both regions. The cells responsible for VEGF expression were fibroblasts and macrophages in the peritumor region or in the pseudocapsule and neoplastic cells in the intratumor region. Greater intratumoral VEGF was expressed in hemangiopericytomas (P = 0.04). No difference was present in the volume fraction of tumor microvessels between VEGF-positive and VEGF-negative neoplasms (P = 0.3416) or for the different types of neoplasms (P = 0.5). The results of this study suggest that VEGF participates in the angiogenesis of soft-tissue sarcoma in dogs. Additional research will be necessary to elucidate the contribution of VEGF to the progression of malignancy.
本研究的目的是评估血管内皮生长因子（VEGF）的表达及犬软组织肉瘤中的微血管密度。使用抗VEGF小鼠单克隆抗体（ab - 119），通过链霉亲和素 - 生物素 - 过氧化物酶法对20例犬肿瘤进行VEGF表达的免疫组织化学检测。在苏木精和伊红染色的组织切片中对肉瘤内微血管的体积分数进行定量。通过放置一个有100个点的网格并计数落入交点的微血管，对每个肿瘤至少10个视野（40倍放大）进行分析。该百分比被视为肿瘤切片中这些微血管的体积分数。在65%的肿瘤中检测到VEGF表达。在92.3%的肿瘤中，表达出现在肿瘤周围区域；在46.15%的肿瘤中，出现在肿瘤内区域；在38.46%的肿瘤中，两个区域均有表达。负责VEGF表达的细胞在肿瘤周围区域或假包膜中是成纤维细胞和巨噬细胞，在肿瘤内区域是肿瘤细胞。血管外皮细胞瘤中肿瘤内VEGF表达更高（P = 0.04）。VEGF阳性和VEGF阴性肿瘤之间肿瘤微血管的体积分数无差异（P = 0.3416），不同类型肿瘤之间也无差异（P = 0.5）。本研究结果表明，VEGF参与犬软组织肉瘤的血管生成。需要进一步研究以阐明VEGF对恶性肿瘤进展的作用。
