Department of Radiology, Tongji Hospital, Tongji University, Shanghai, China.
Dement Geriatr Cogn Disord. 2009;28(6):558-66. doi: 10.1159/000261646. Epub 2010 Jan 6.
BACKGROUND/AIMS: To explore the potential value of myo-inositol (mIns), which is regarded as a biomarker for early diagnosis of Alzheimer's disease, in APP/PS1 transgenic (tg) mice detected by (1)H-MRS.
(1)H-MRS was performed in 30 APP/PS1 tg mice and 20 wild-type (wt) littermates at 3, 5 and 8 months of age. Areas under the peak of N-acetylaspartate (NAA), mIns and creatine (Cr) in the frontal cortex and hippocampus were measured, and the NAA/Cr and mIns/Cr ratios were analyzed quantitatively.
Compared with the wt mice, the mIns/Cr ratio of the 3-month-old tg mice was significantly higher (p < 0.05), and pathology showed activation and proliferation of astrocytes in the frontal cortex and hippocampus. The concentration of NAA was significantly lower at 8 and 8 months of age (p < 0.05). According to the threshold of mIns/Cr that was adopted to separate the tg from the wt mice, the rate of correct predictions was 82, 94 and 95%, respectively, for 3, 5 and 8 months.
Of the early AD metabolites as detected by (1)H-MRS, mIns is the most valuable marker for assessment of AD. Quantitative analysis of mIns may provide important clues for early diagnosis of AD.
背景/目的：探索肌醇（mIns）作为阿尔茨海默病早期诊断生物标志物的潜在价值，通过（1）H-MRS 在 APP/PS1 转基因（tg）小鼠中进行检测。
对 30 只 APP/PS1 tg 小鼠和 20 只野生型（wt）同窝仔鼠在 3、5 和 8 月龄时进行（1）H-MRS 检测。测量额皮质和海马区中 N-乙酰天冬氨酸（NAA）、mIns 和肌酸（Cr）峰下面积，并进行定量分析 NAA/Cr 和 mIns/Cr 比值。
与 wt 小鼠相比，3 月龄 tg 小鼠的 mIns/Cr 比值显著升高（p < 0.05），病理显示额皮质和海马区的星形胶质细胞活化和增殖。8 月龄和 8 月龄时 NAA 浓度显著降低（p < 0.05）。根据将 tg 与 wt 小鼠区分开来的 mIns/Cr 阈值，3、5 和 8 个月时的正确预测率分别为 82%、94%和 95%。
在（1）H-MRS 检测的早期 AD 代谢物中，mIns 是评估 AD 的最有价值的标志物。mIns 的定量分析可能为 AD 的早期诊断提供重要线索。
