Kyriakopoulou Zaharoula, Dedepsidis Evaggelos, Pliaka Vaia, Mastorakos Panayotis, Stamati Anastassia, Pratti Anastassia, Levidiotou-Stefanou Stamatina, Markoulatos Panayotis
Department of Biochemistry & Biotechnology, University of Thessaly, 26 Ploutonos & Aeolou Str, Larissa 41221, Greece.
Virus Genes. 2010 Apr;40(2):183-92. doi: 10.1007/s11262-009-0446-y. Epub 2010 Jan 22.
Two enteroviruses from river water and four from sewage treatment plant were isolated in Larissa, Greece, that all shared the same sequence. A full genome analysis was conducted in an attempt to reveal the evolutionary pathways of one of the isolated strains (LR11F7). VP1 nucleotide and phylogenetic analysis revealed that the isolated strain had 78% homology with the echovirus 7 prototype strain Wallace. Full genome analysis revealed that LR11F7 P1 region is related to echoviruses 7 and that P2 and P3 regions are originating from contemporary enteroviruses isolated in South Asia. Two recombination events were shown to be involved into the evolutionary history of LR11F7, the one event concerning 3A, 3B, and 2C, and the other concerning 3D genomic region, both with new types of HEV-B. The contribution of recombination to enterovirus evolution is substantial, giving rise to new genetic lineages with unknown properties.
在希腊拉里萨，从河水中分离出两种肠道病毒，从污水处理厂分离出四种肠道病毒，它们都具有相同的序列。对其中一株分离株（LR11F7）进行了全基因组分析，以揭示其进化途径。VP1核苷酸和系统发育分析表明，分离株与埃可病毒7原型株华莱士具有78%的同源性。全基因组分析表明，LR11F7的P1区域与埃可病毒7相关，P2和P3区域源自南亚分离出的当代肠道病毒。研究表明，LR11F7的进化历史涉及两次重组事件，一次涉及3A、3B和2C，另一次涉及3D基因组区域，均与新型戊型肝炎病毒B有关。重组对肠道病毒进化的贡献很大，产生了具有未知特性的新遗传谱系。
