Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
Clin Microbiol Infect. 2011 Aug;17(8):1170-3. doi: 10.1111/j.1469-0691.2010.03381.x. Epub 2011 Apr 12.
The molecular characterization of two enterovirus strains (LR51A5 and LR61G3) isolated from the sewage treatment plant unit in Larissa, Greece, in May and June 2006 and the investigation of their relationship with enteroviruses of the same serotype isolated in Greece in 2001 and 2007 were performed by complete VP1 sequence analysis of the isolates. The close phylogenetic relationship and the high nucleotide similarity (98%) led to the conclusion that the virus isolated from sewage in 2006 was associated with that isolated from an aseptic meningitis outbreak 1 year later. Bootscan analysis of the VP1 genomic region revealed that intraserotypic multi-recombination events might have been involved in the evolutionary past history of the LR51A5 and LR61G3 isolates.
通过对 2006 年 5 月和 6 月从希腊拉里萨污水处理厂单元分离的两种肠病毒株（LR51A5 和 LR61G3）进行完整 VP1 序列分析，对其进行了分子特征描述，并研究了它们与 2001 年和 2007 年在希腊分离的同血清型肠病毒的关系。密切的系统发育关系和高核苷酸相似性（98%）得出结论，2006 年从污水中分离出的病毒与 1 年后无菌性脑膜炎爆发中分离出的病毒有关。VP1 基因组区域的 Bootscan 分析表明，同种型内的多次重组事件可能参与了 LR51A5 和 LR61G3 分离株的进化史。
