Chongsiriwatana Nathaniel P, Barron Annelise E
Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.
Methods Mol Biol. 2010;618:171-82. doi: 10.1007/978-1-60761-594-1_12.
Interactions with bacterial membranes are integral to the mechanisms of action of all antimicrobial peptides (AMPs), regardless of their final cellular targets. Here, we describe in detail two biophysical techniques that can be used to measure the membrane activities of AMPs and antimicrobial peptidomimetics: (1) a calcein leakage assay to investigate interactions between AMPs/peptidomimetics with large unilamellar vesicles and (2) a potential-sensitive dye-based depolarization assay to investigate interactions with the membranes of live bacteria. By comparing the membrane interactions of AMPs and their mimics, these techniques can provide insights into their extent of mimicry and their antimicrobial mechanisms.
与细菌膜的相互作用是所有抗菌肽（AMPs）作用机制的组成部分，无论其最终的细胞靶点是什么。在这里，我们详细描述两种可用于测量AMPs和抗菌肽模拟物膜活性的生物物理技术：（1）一种钙黄绿素泄漏测定法，用于研究AMPs/肽模拟物与大单层囊泡之间的相互作用；（2）一种基于电位敏感染料的去极化测定法，用于研究与活细菌膜的相互作用。通过比较AMPs及其模拟物的膜相互作用，这些技术可以深入了解它们的模拟程度及其抗菌机制。
