Institut de Physiologie et Biologie Cellulaires, Université de Poitiers, Centre National de la Recherche Scientifique (CNRS), Poitiers, France.
Hippocampus. 2011 Mar;21(3):233-8. doi: 10.1002/hipo.20765.
Adult neurogenesis mainly occurs in two brain regions, the subventricular zone and the dentate gyrus (DG) of the hippocampus. Neuropeptide Y (NPY) is widely expressed throughout the brain and is known to enhance in vitro hippocampal cell proliferation. Mice lacking either NPY or the Y1 receptor display lower levels of cell proliferation, thereby suggesting a role for NPY in basal in vivo neurogenesis. Here, we investigated whether exogenous NPY stimulates DG progenitors proliferation in vivo. We show that intracerebroventricular administration of NPY increases DG cell proliferation and promotes neuronal differentiation in C57BL/6 adult mice. In these mice, the proliferative effect of NPY is mediated by the Y1 and not the Y2 receptor, as a Y1 ([Leu(31) ,Pro(34) ]), but not a Y2 (NPY(3-36) ), receptor agonist enhanced proliferation. In addition, no NPY-induced DG cellular proliferation is observed following NPY injection when coadministered with a Y1 antagonist or in the Y1 receptor knockout mouse. These results are in line with data obtained in Y1(-/-) mice, demonstrating that NPY regulates in vivo hippocampal neurogenesis. © 2010 Wiley-Liss, Inc.
成年神经发生主要发生在两个脑区，即侧脑室下区和海马齿状回（DG）。神经肽 Y（NPY）广泛表达于整个大脑，已知其可增强海马体培养细胞的增殖。缺乏 NPY 或 Y1 受体的小鼠显示出较低水平的细胞增殖，从而表明 NPY 在基础的体内神经发生中发挥作用。在这里，我们研究了外源性 NPY 是否会刺激体内 DG 祖细胞的增殖。我们发现，脑室给药 NPY 可增加 C57BL/6 成年小鼠 DG 细胞的增殖并促进其神经元分化。在这些小鼠中，NPY 的增殖作用是由 Y1 而不是 Y2 受体介导的，因为 Y1（[Leu（31），Pro（34）]）而非 Y2（NPY（3-36））受体激动剂可增强增殖。此外，当与 Y1 拮抗剂共给药或在 Y1 受体敲除小鼠中，NPY 注射后不会观察到 DG 细胞增殖。这些结果与在 Y1（-/-）小鼠中获得的数据一致，表明 NPY 调节体内海马神经发生。©2010 年 Wiley-Liss，Inc.
