Gaukler Shannon M, Linz George M, Sherwood Julie S, Dyer Neil W, Bleier William J, Wannemuehler Yvonne M, Nolan Lisa K, Logue Catherine M
Department of Biological Sciences, North Dakota State University, Fargo, ND 58108, USA.
Avian Dis. 2009 Dec;53(4):544-51. doi: 10.1637/8920-050809-Reg.1.
The prevalence of Escherichia coli, Salmonella spp., and Mycobacterium avium subsp. paratuberculosis isolated from the feces of wild European starlings (Sturnus vulgaris) humanely trapped at a feedlot in central Kansas was assessed. All E. coli and Salmonella isolates recovered were tested for antimicrobial susceptibility using National Antimicrobial Resistance Monitoring System panels and the E. coli isolates were classified as to their content of genes associated with pathogenic E. coli of birds and cattle, including cvaC, iroN2, ompTp, hlyF2, eitC, iss, iutA, ireA, papC, stxI, stxII, sta, K99, F41, and eae. Escherichia coli O157:H7 and Mycobacterium avium subsp. paratuberculosis were not detected and Salmonella was isolated from only three samples, two of which displayed antimicrobial resistance. Approximately half of the E. coli isolates were resistant to antimicrobial agents with 96% showing resistance to tetracycline. Only one isolate was positive for a single gene associated with bovine pathogenic E. coli. An interesting finding of this study was that 5% of the E. coli isolates tested met the criteria established for identification as avian pathogenic E. coli (APEC). Thus these findings suggest that starlings are not a significant source of Salmonella spp., Mycobacterium avium subsp. paratuberculosis, E. coli O157, or other shiga toxin-producing E. coli in this feedlot. However, they may have the potential to spread APEC, an important pathogen of poultry and a potential pathogen to human beings.
对在堪萨斯州中部一个饲养场中被人道捕获的野生欧洲椋鸟（家八哥）粪便中分离出的大肠杆菌、沙门氏菌和副结核分枝杆菌的流行情况进行了评估。使用国家抗菌药物耐药性监测系统面板对所有回收的大肠杆菌和沙门氏菌分离株进行了抗菌药物敏感性测试，并对大肠杆菌分离株进行了与鸟类和牛的致病性大肠杆菌相关基因含量的分类，包括cvaC、iroN2、ompTp、hlyF2、eitC、iss、iutA、ireA、papC、stxI、stxII、sta、K99、F41和eae。未检测到大肠杆菌O157:H7和副结核分枝杆菌，仅从三个样本中分离出沙门氏菌，其中两个显示出抗菌药物耐药性。大约一半的大肠杆菌分离株对抗菌药物耐药，96%对四环素耐药。只有一个分离株对与牛致病性大肠杆菌相关的单个基因呈阳性。这项研究的一个有趣发现是，5%的测试大肠杆菌分离株符合被鉴定为禽致病性大肠杆菌（APEC）的既定标准。因此，这些发现表明椋鸟不是该饲养场中沙门氏菌、副结核分枝杆菌、大肠杆菌O157或其他产志贺毒素大肠杆菌的重要来源。然而，它们可能有传播APEC的潜力，APEC是家禽的一种重要病原体，也是人类的潜在病原体。
