Masuda T, Beier K, Yamamoto K, Fahimi H D
Department of Anatomy and Cell Biology II, University of Heidelberg, Federal Republic of Germany.
Cell Tissue Res. 1991 Jan;263(1):145-54. doi: 10.1007/BF00318410.
We have studied the ultrastructural characteristics and the distribution of peroxisomes in guinea pig liver using electron-microscopic cytochemistry for catalase and morphometry. By light microscopy, peroxisomes appear as dark 0.2-0.5 microns granules in the cytoplasm of liver parenchymal cells, often forming large clusters that measure up to 5 microns across. Rows of single peroxisomes or their aggregates line the sinusoidal surface of hepatocytes. Electron microscopy reveals that clusters of up to 25 individual peroxisomes are usually located in the subsinusoidal region of parenchymal cells. The mean diameter and the volume density of peroxisomes are larger in pericentral than in periportal regions of the liver lobule. Whereas large amounts of lipoprotein particles with a mean diameter of 160 nm (chylomicrons) are present in the Disse space, the cytoplasm of parenchymal cells contains multivesicular bodies and abundant lipid droplets. In addition, the Golgi complexes show distended lipoprotein-filled vesicles suggesting active biosynthesis of lipoproteins. We propose that the unique features of peroxisomes in guinea pig liver, such as cluster formation and alignment along the sinusoidal surface, may be related to the high levels of lipoproteins in the portal circulation and their hepatic catabolism in this species.
我们运用过氧化氢酶的电子显微镜细胞化学技术和形态计量学方法，研究了豚鼠肝脏中过氧化物酶体的超微结构特征及其分布。通过光学显微镜观察，过氧化物酶体在肝实质细胞的细胞质中呈现为直径0.2 - 0.5微米的深色颗粒，常常聚集成直径达5微米的大簇。单行排列的单个过氧化物酶体或其聚集体排列在肝细胞的窦状表面。电子显微镜显示，通常有多达25个单个过氧化物酶体的簇位于实质细胞的窦周区域。肝小叶中央周围区域的过氧化物酶体平均直径和体积密度比门周区域的大。在狄氏间隙中存在大量平均直径为160纳米的脂蛋白颗粒（乳糜微粒），实质细胞的细胞质中含有多囊泡体和丰富的脂滴。此外，高尔基体显示出充满脂蛋白的扩张囊泡，提示脂蛋白的活跃生物合成。我们推测，豚鼠肝脏中过氧化物酶体的独特特征，如簇的形成以及沿窦状表面排列，可能与门脉循环中高水平的脂蛋白及其在该物种中的肝脏分解代谢有关。
