Cai W Q, Bodin P, Sexton A, Loesch A, Burnstock G
Department of Anatomy and Developmental Biology, University College London, United Kingdom.
Cell Tissue Res. 1993 Apr;272(1):175-81. doi: 10.1007/BF00323584.
The localization of neuropeptide Y (NPY) and atrial natriuretic peptide (ANP) in the endothelial cells of human umbilical blood vessels was studied using the pre-embedding peroxidase-antiperoxidase (PAP) technique for electron microscopy and avidin-biotin-complex (ABC) immunostaining for endothelial cells cultured from umbilical vein. Subpopulations of NPY- and ANP-immunoreactive endothelial cells were present in term umbilical vein and artery. The umbilical vein contained more positive cells than the artery. The percentage of NPY- and ANP-immunoreactive umbilical vein cells in culture was 32% and 44%, respectively, out of a total of 3013 cells examined. The possibility that these potent vasoactive substances located in the endothelial cells of the non-innervated umbilical vessels are involved in the local regulation of blood flow is discussed.
利用预包埋过氧化物酶-抗过氧化物酶（PAP）技术进行电子显微镜观察，并采用抗生物素蛋白-生物素复合物（ABC）免疫染色法对人脐静脉培养的内皮细胞进行研究，以探讨神经肽Y（NPY）和心钠素（ANP）在人脐血管内皮细胞中的定位。足月脐静脉和动脉中存在NPY和ANP免疫反应性内皮细胞亚群。脐静脉中的阳性细胞比动脉中的多。在总共检查的3013个细胞中，培养的脐静脉细胞中NPY和ANP免疫反应性细胞的百分比分别为32%和44%。本文讨论了这些位于无神经支配的脐血管内皮细胞中的强效血管活性物质参与局部血流调节的可能性。
