Faculty of Health Care, Kiryu University, Midori, Gunma, Japan.
Pancreas. 2010 Jul;39(5):627-32. doi: 10.1097/MPA.0b013e3181c52bc5.
We previously reported that vagal hyperactivity produced by ventromedial hypothalamic (VMH) lesions stimulated cell proliferation of rat pancreatic islet B and acinar cells primarily through a cholinergic receptor mechanism.
This study examined how gene families involved in cell proliferation are regulated after VMH lesions formation. Total pancreatic RNA was extracted, and differences in the gene expression profiles between rats at day 3 after VMH lesioning and sham-VMH-lesioned rats were investigated using DNA microarray and real-time polymerase chain reaction.
The VMH lesions regulated the genes that are involved in functions related to cellular growth and proliferation and neuronal development in the pancreas. Real-time polymerase chain reaction also confirmed that gene expressions of angiotensin II receptor-like 1 (AGTRL1) and proline rich 15 (PRR15) were down-regulated at day 3 after the VMH lesions.
Ventromedial hypothalamic lesions may change the expression of cell proliferation-related genes and neuron-related genes in a rat pancreas.
我们之前报道过，腹内侧下丘脑（VMH）损伤引起的迷走神经活性亢进主要通过胆碱能受体机制刺激大鼠胰岛 B 和腺泡细胞的细胞增殖。
本研究探讨了 VMH 损伤形成后参与细胞增殖的基因家族是如何被调控的。提取总胰腺 RNA，利用 DNA 微阵列和实时聚合酶链反应比较 VMH 损伤后 3 天大鼠和假 VMH 损伤大鼠的基因表达谱差异。
VMH 损伤调节了与胰腺细胞生长和增殖以及神经元发育相关的基因。实时聚合酶链反应也证实，血管紧张素 II 受体样 1（AGTRL1）和富含脯氨酸 15（PRR15）的基因表达在 VMH 损伤后 3 天下调。
VMH 损伤可能会改变大鼠胰腺中与细胞增殖和神经元相关的基因表达。
