Department of Applied Molecular Bioscience, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan.
Biochem Biophys Res Commun. 2010 Feb 26;393(1):16-20. doi: 10.1016/j.bbrc.2010.01.063. Epub 2010 Jan 25.
The gene of (all-E) geranylfarnesyl diphosphate synthase that is responsible for the biosynthesis of methanophenazine, an electron carrier utilized for methanogenesis, was cloned from a methanogenic archaeon Methanosarcina mazei Gö1. The properties of the recombinant enzyme and the results of phylogenetic analysis suggest that the enzyme is closely related to (all-E) prenyl diphosphate synthases that are responsible for the biosynthesis of respiratory quinones, rather than to the enzymes involved in the biosynthesis of archaeal membrane lipids, including (all-E) geranylfarnesyl diphosphate synthase from a thermophilic archaeon.
从产甲烷古菌 Methanosarcina mazei Gö1 中克隆了负责甲烷吩嗪生物合成的（全-E）香叶基二磷酸合酶基因，甲烷吩嗪是一种用于甲烷生成的电子载体。重组酶的特性和系统发育分析的结果表明，该酶与负责呼吸醌生物合成的（全-E） prenyl diphosphate 合酶密切相关，而不是与参与古菌膜脂生物合成的酶有关，包括来自嗜热古菌的（全-E）香叶基二磷酸合酶。
Zotero 插件