Department of Infection and Immunity, The University of Sheffield, Sheffield, UK.
Hum Immunol. 2010 Apr;71(4):355-62. doi: 10.1016/j.humimm.2010.01.019. Epub 2010 Feb 19.
Common variable immunodeficiency (CVID) is a heterogeneous group of disorders, characterized by hypogammaglobulinemia and normal or low numbers of B cells, which predispose patients to recurrent infections. Peripheral blood mononuclear cells from 19 patients with CVID, and age- and sex-matched controls, were subjected to an in vitro assay of B-cell-T-cell activation and interaction, using anti-immunoglobulin (Ig)-D conjugated to dextran (alpha-delta-dex), as a polyclonal T independent type 2 antigen mimic, with and without anti-CD3/anti-CD28, as polyclonal T-cell stimuli. Stimulation of lymphocytes with either anti-CD3 or anti-CD3 plus anti-CD28 induced T-cell activation and proliferation in CVID patients who were similar to age- and sex-matched controls, but B cells of patients were significantly less activated when peripheral blood mononuclear cells were stimulated with polyclonal T-cell agonists alone. Comparison of CD86 expression in the patients with matched controls revealed that patients had low B-cell activation in response to T-cell stimuli (bystander T-cell help). In conclusion, this sample of CVID patients exhibits a defect of T-cell "help" to B cells, and/or B-cell response to T-cell help.
普通变异性免疫缺陷症（CVID）是一组异质性疾病，其特征为低丙种球蛋白血症和正常或低数量的 B 细胞，这使患者容易发生反复感染。对 19 名 CVID 患者和年龄、性别匹配的对照者的外周血单个核细胞进行 B 细胞-T 细胞激活和相互作用的体外检测，使用与葡聚糖（alpha-delta-dex）偶联的抗免疫球蛋白（Ig）-D 作为多克隆 T 非依赖性 2 型抗原模拟物，有和没有抗-CD3/抗-CD28，作为多克隆 T 细胞刺激物。用抗-CD3 或抗-CD3 加抗-CD28 刺激淋巴细胞可诱导 CVID 患者的 T 细胞激活和增殖，与年龄和性别匹配的对照者相似，但当单独用多克隆 T 细胞激动剂刺激外周血单个核细胞时，患者的 B 细胞明显激活减少。与匹配的对照者比较 CD86 的表达表明，患者对 T 细胞刺激的 B 细胞激活不足（旁观者 T 细胞辅助）。总之，本 CVID 患者样本显示 T 细胞对 B 细胞的“辅助”存在缺陷，和/或 B 细胞对 T 细胞辅助的反应存在缺陷。
