Biotechnology Research Division, National Fisheries Research and Development Institute, Gijang, Busan 619-705, Republic of Korea.
Korea Research Institute of Bioscience and Biotechnology, PO Box 115, Yusong, Taejon, Republic of Korea.
Int J Syst Evol Microbiol. 2010 Dec;60(Pt 12):2908-2912. doi: 10.1099/ijs.0.021808-0. Epub 2010 Jan 22.
A Gram-stain-negative, non-motile, non-spore-forming and short rod- or rod-shaped bacterial strain, designated 22-5(T), was isolated from a bluespotted cornetfish, Fistularia commersonii, and subjected to taxonomic study. Strain 22-5(T) grew optimally at 30 °C and in the presence of 2-5 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain 22-5(T) belonged to the genus Paracoccus and joined the cluster comprising Paracoccus homiensis DD-R11(T) and Paracoccus zeaxanthinifaciens ATCC 21588(T), with which strain 22-5(T) exhibited 97.4 and 96.9 % 16S rRNA gene sequence similarity, respectively. Strain 22-5(T) exhibited 94.0-96.6 % 16S rRNA gene sequence similarity with the other type strains of species of the genus Paracoccus. Strain 22-5(T) contained Q-10 as the predominant menaquinone and C(18 : 1)ω7c as the predominant fatty acid. In this study, P. zeaxanthinifaciens KCTC 22688(T) also contained Q-10 as the predominant isoprenoid quinone. The DNA G+C content of strain 22-5(T) was 63.6 mol%. Strain 22-5(T) exhibited 44 and 32 % DNA-DNA relatedness to P. homiensis KACC 11518(T) and P. zeaxanthinifaciens KCTC 22688(T), respectively. On the basis of phenotypic, phylogenetic and genetic data, strain 22-5(T) is considered to represent a novel species of the genus Paracoccus, for which the name Paracoccus fistulariae sp. nov. is proposed. The type strain is 22-5(T) (=KCTC 22803(T) =CCUG 58401(T)).
一株革兰氏阴性、非运动、非孢子形成的短杆状或杆状细菌菌株，被命名为 22-5(T)，从蓝斑笛鲷（Fistularia commersonii）中分离出来，并进行了分类学研究。菌株 22-5(T) 在 30 °C 和 2-5%（w/v）NaCl 的存在下生长最佳。基于 16S rRNA 基因序列的系统发育分析表明，菌株 22-5(T) 属于副球菌属，与副球菌属的 Paracoccus homiensis DD-R11(T) 和 Paracoccus zeaxanthinifaciens ATCC 21588(T) 聚类在一起，与这两个菌株的 16S rRNA 基因序列相似度分别为 97.4%和 96.9%。菌株 22-5(T) 与副球菌属的其他种的模式菌株的 16S rRNA 基因序列相似度为 94.0-96.6%。菌株 22-5(T) 含有 Q-10 作为主要的menaquinone 和 C(18 : 1)ω7c 作为主要的脂肪酸。在本研究中，Paracoccus zeaxanthinifaciens KCTC 22688(T) 也含有 Q-10 作为主要的异戊烯醌。菌株 22-5(T) 的 DNA G+C 含量为 63.6 mol%。菌株 22-5(T) 与 Paracoccus homiensis KACC 11518(T) 和 Paracoccus zeaxanthinifaciens KCTC 22688(T) 的 DNA-DNA 相关性分别为 44%和 32%。基于表型、系统发育和遗传数据，菌株 22-5(T) 被认为代表副球菌属的一个新种，命名为 Paracoccus fistulariae sp. nov.。模式菌株为 22-5(T)=KCTC 22803(T)=CCUG 58401(T)。
