Park Sooyeon, Choi Jeehyun, Choi Su Jung, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Nov;67(11):4760-4766. doi: 10.1099/ijsem.0.002373. Epub 2017 Oct 6.
A Gram-stain-negative, non-spore-forming, non-motile and coccoid, ovoid or short rod-shaped bacterial strain, designated GHD-05, was isolated from a tidal flat on the Yellow Sea in South Korea. Strain GHD-05 grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain GHD-05 belonged to the genus Paracoccus, clustering with the type strain of Paracoccusaestuariivivens. Strain GHD-05 exhibited 16S rRNA gene sequence similarity values of 97.0-99.1 % to the type strains of Paracoccusaestuariivivens, Paracoccuslimosus, Paracoccuslaeviglucosivorans and Paracoccusmarinus, and of 94.2-96.9 % to the type strains of the other Paracoccus species. Strain GHD-05 contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids detected in strain GHD-05 were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminolipid and one unidentified glycolipid. The DNA G+C content of strain GHD-05 was 64.1 mol% and its DNA-DNA relatedness values with the type strains of P. aestuariivivens, Paracoccuslimosus, P. laeviglucosivorans and P. marinus were 13-32 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain GHD-05 is separated from recognized Paracoccus species. On the basis of the data presented here, strain GHD-05 is considered to represent a novel species of the genus Paracoccus, for which the name Paracoccuslitorisediminis sp. nov. is proposed. The type strain is GHD-05 (=KCTC 52978=NBRC 112902).
从韩国黄海潮间带分离出一株革兰氏阴性、不形成芽孢、无运动性、呈球状、卵形或短杆状的细菌菌株，命名为GHD-05。菌株GHD-05在30°C、pH 7.0 - 8.0以及2.0%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的系统发育树表明，菌株GHD-05属于副球菌属，与河口副球菌模式菌株聚类。菌株GHD-05与河口副球菌、海滨副球菌、光滑葡萄糖副球菌和海生副球菌模式菌株的16S rRNA基因序列相似性值为97.0 - 99.1%，与其他副球菌属模式菌株的相似性值为94.2 - 96.9%。菌株GHD-05以Q-10作为主要泛醌，以C18 : 1ω7c作为主要脂肪酸。在菌株GHD-05中检测到的主要极性脂为磷脂酰胆碱、磷脂酰甘油、双磷脂酰甘油、一种未鉴定的氨基脂和一种未鉴定的糖脂。菌株GHD-05的DNA G+C含量为64.1 mol%，其与河口副球菌、海滨副球菌、光滑葡萄糖副球菌和海生副球菌模式菌株的DNA-DNA相关性值为13 - 32%。不同的表型特征，以及其系统发育和遗传独特性，表明菌株GHD-05与已认可的副球菌属物种不同。基于本文提供的数据，菌株GHD-05被认为代表副球菌属的一个新物种，为此提议命名为海滨副球菌缩减亚种（Paracoccus litorisediminis sp. nov.）。模式菌株为GHD-05（=KCTC 52978=NBRC 112902）。
