Research and Development Division, H-Plus Eco Ltd, BVC #301, KRIBB, Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
School of Civil and Environmental Engineering, Yonsei University, Seoul 120-749, Republic of Korea.
Int J Syst Evol Microbiol. 2011 Aug;61(Pt 8):1968-1972. doi: 10.1099/ijs.0.017897-0. Epub 2010 Sep 17.
A Gram-negative, non-motile bacterium, designated MJ17(T), was isolated from sludge at the Daejeon sewage disposal plant in South Korea. Comparative 16S rRNA gene sequence analysis showed that strain MJ17(T) belonged to the genus Paracoccus in the family Rhodobacteraceae of the class Alphaproteobacteria. 16S rRNA gene sequence similarities between strain MJ17(T) and type strains of species of the genus Paracoccus were 94.1-97.4 %. The highest similarities were between strain MJ17(T) and Paracoccus homiensis DD-R11(T), Paracoccus zeaxanthinifaciens ATCC 21588(T) and Paracoccus alcaliphilus JCM 7364(T) (97.4, 97.2 and 96.3 %, respectively). Strain MJ17(T) exhibited <22 % DNA-DNA relatedness with P. homiensis KACC 11518(T) and P. zeaxanthinifaciens JCM 21774(T). The G+C content of the genomic DNA was 58.7 mol%. Strain MJ17(T) contained ubiquinone Q-10. The major fatty acids were C(18 : 0) (11.3 %), C(16 : 0) (10.2 %) and summed feature 7 (containing one or more of C(18 : 1)ω7c, C(18 : 1)ω9c and C(18 : 1)ω12t; 54.3 %). Poly-β-hydroxybutyrate granules are formed. On the basis of phenotypic and genotypic properties and phylogenetic distinctiveness, strain MJ17(T) should be classified in a novel species of the genus Paracoccus, for which the name Paracoccus caeni sp. nov. is proposed. The type strain is MJ17(T) ( = KCTC 22480(T) = JCM 16385(T) = KEMB 9004-001(T)).
一株革兰氏阴性、非运动性细菌，被命名为 MJ17(T)，从韩国大田污水处理厂的污泥中分离得到。比较 16S rRNA 基因序列分析表明，菌株 MJ17(T)属于红杆菌科副球菌属的α变形菌纲。菌株 MJ17(T)与副球菌属种的模式菌株的 16S rRNA 基因序列相似性为 94.1-97.4%。与菌株 MJ17(T)相似度最高的是 Paracoccus homiensis DD-R11(T)、Paracoccus zeaxanthinifaciens ATCC 21588(T)和 Paracoccus alcaliphilus JCM 7364(T)（分别为 97.4%、97.2%和 96.3%）。菌株 MJ17(T)与 Paracoccus homiensis KACC 11518(T)和 Paracoccus zeaxanthinifaciens JCM 21774(T)的 DNA-DNA 相关性<22%。基因组 DNA 的 G+C 含量为 58.7mol%。菌株 MJ17(T)含有泛醌 Q-10。主要脂肪酸为 C(18:0)（11.3%）、C(16:0)（10.2%）和总和特征 7（包含一个或多个 C(18:1)ω7c、C(18:1)ω9c 和 C(18:1)ω12t；54.3%）。形成聚-β-羟基丁酸颗粒。基于表型和基因型特性以及系统发育的独特性，菌株 MJ17(T)应归类为副球菌属的一个新种，命名为 Paracoccus caeni sp. nov.。模式菌株为 MJ17(T)（=KCTC 22480(T)=JCM 16385(T)=KEMB 9004-001(T)）。
