Amara Salem, Garrel Catherine, Favier Alain, Ben Rhouma Khémais, Sakly Mohsen, Abdelmelek Hafedh
Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, 7021 Jarzouna, Tunisia.
Gen Physiol Biophys. 2009 Dec;28(4):414-9. doi: 10.4149/gpb_2009_04_414.
Currently, environmental and industrial pollution along with increase and causes multiple stress conditions, the combined exposure to magnetic field and other toxic agents is recognised as an important research area, with a view to better protecting human health against their probable unfavourable effects. In the present study, we investigated the effect of co-exposure to static magnetic field (SMF) and cadmium (Cd) on the antioxidant enzymes activity and the malondialdehyde (MDA) concentration in rat skeletal and cardiac muscles. The exposure of rats to SMF (128 mT, 1 h/day during 30 consecutive days) decreased the activities of glutathione peroxidase (GPx) and the superoxide dismutase (CuZn-SOD) in heart muscle. Sub-chronic exposure to SMF increased the MDA concentration in rat cardiac muscle. Cd treatment (CdCl2, 40 mg/l, per os) during 4 weeks decreased the activities of catalase (CAT) in skeletal muscle and the CuZn-SOD in the heart. Moreover, Cd administration increased MDA concentration in the both structures. The combined effect of SMF (128 mT, 1 h/day during 30 consecutive days) and Cd (40 mg/l, per os) disrupt the antioxidant enzymes activity in rat skeletal and cardiac muscles. Moreover, we noted a huge increase in MDA concentration in the heart and skeletal muscle compared to control group. Thus it is possible that the SMF- and/or Cd-induced depletion of antioxidant enzymes activity in muscle tissues might, like the enhanced lipid peroxidation, importantly contribute to oxidative damage. The combined effect of SMF and Cd altered significantly the antioxidant enzymatic capacity and induced lipid peroxidation in both skeletal and cardiac muscle.
目前，环境和工业污染以及其他因素增加并导致了多种应激状况，磁场与其他有毒物质的联合暴露被视为一个重要的研究领域，旨在更好地保护人类健康免受其可能产生的不利影响。在本研究中，我们调查了静态磁场（SMF）和镉（Cd）共同暴露对大鼠骨骼肌和心肌中抗氧化酶活性以及丙二醛（MDA）浓度的影响。将大鼠暴露于SMF（128 mT，连续30天每天1小时）会降低心肌中谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（CuZn-SOD）的活性。亚慢性暴露于SMF会增加大鼠心肌中的MDA浓度。4周内口服Cd处理（CdCl2，40 mg/l）会降低骨骼肌中的过氧化氢酶（CAT）活性以及心脏中的CuZn-SOD活性。此外，给予Cd会增加这两种组织中的MDA浓度。SMF（128 mT，连续30天每天1小时）和Cd（40 mg/l，口服）的联合作用会破坏大鼠骨骼肌和心肌中的抗氧化酶活性。此外，我们注意到与对照组相比，心脏和骨骼肌中的MDA浓度大幅增加。因此，肌肉组织中SMF和/或Cd诱导的抗氧化酶活性耗竭可能与脂质过氧化增强一样，对氧化损伤有重要影响。SMF和Cd的联合作用显著改变了抗氧化酶能力，并在骨骼肌和心肌中诱导了脂质过氧化。
