静磁场暴露对大鼠脑组织抗氧化酶活性及DNA的影响。

Effects of static magnetic field exposure on antioxidative enzymes activity and DNA in rat brain.

作者信息

Amara Salem, Douki Thierry, Garel Catherine, Favier Alain, Sakly Mohsen, Rhouma Khémais B, Abdelmelek Hafedh

机构信息

Laboratoire de Physiologie Animale, Faculté des Sciences de Bizerte, 7021 Jarzouna, Tunisia.

出版信息

Gen Physiol Biophys. 2009 Sep;28(3):260-5. doi: 10.4149/gpb_2009_03_260.

DOI:10.4149/gpb_2009_03_260

PMID:20037191

Abstract

The present study was undertaken in order to investigate the effects of static magnetic field (SMF) exposure on the antioxidative enzymes activity, malondialdehyde (MDA) concentration and DNA oxidation in male rat brain. The exposure of rats to SMF (128 mT, 1 h/day during 30 consecutive days) decreased the glutathione peroxidase (GPx; -39%, p < 0.05), CuZn superoxide dismutase (CuZn-SOD; -35%, p < 0.05) and catalase (-59%, p < 0.05) activities in frontal cortex. The same treatment decreased the CuZn-SOD (-51%, p < 0.05) and Mn-SOD (-13%, p < 0.05) activities in hippocampus. However, the glutathione levels remained unchanged in the both brain structures. In the hippocampus, SMF exposure increased MDA concentration (+32%, p < 0.05). Interestingly, exposed-rats to SMF displayed a significant increase of metallothioneins level in frontal cortex (+100%, p < 0.05), while the 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) concentration remained unaffected, indicating the absence of DNA oxidation. Our results indicated that sub-chronic exposure to SMF induced oxidative stress in rat hippocampus and frontal cortex. Metallothionein induction protected probably DNA against oxidative damage.

摘要

本研究旨在探讨静磁场（SMF）暴露对雄性大鼠大脑抗氧化酶活性、丙二醛（MDA）浓度和DNA氧化的影响。将大鼠暴露于SMF（128 mT，连续30天，每天1小时）后，额叶皮质中的谷胱甘肽过氧化物酶（GPx；降低39%，p < 0.05）、铜锌超氧化物歧化酶（CuZn-SOD；降低35%，p < 0.05）和过氧化氢酶（降低59%，p < 0.05）活性降低。相同处理使海马体中的CuZn-SOD（降低51%，p < 0.05）和锰超氧化物歧化酶（Mn-SOD；降低13%，p < 0.05）活性降低。然而，在这两个脑区结构中，谷胱甘肽水平保持不变。在海马体中，SMF暴露使MDA浓度升高（升高32%，p < 0.05）。有趣的是，暴露于SMF的大鼠额叶皮质中的金属硫蛋白水平显著升高（升高100%，p < 0.05），而8-氧代-7,8-二氢-2'-脱氧鸟苷（8-氧代dGuo）浓度未受影响，表明不存在DNA氧化。我们的结果表明，亚慢性暴露于SMF会在大鼠海马体和额叶皮质中诱导氧化应激。金属硫蛋白的诱导可能保护DNA免受氧化损伤。