Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, Jarzouna, Tunisia.
Environ Toxicol Pharmacol. 2011 Jan;31(1):100-6. doi: 10.1016/j.etap.2010.09.010. Epub 2010 Sep 29.
The aim of this study was to investigate the effect of selenium supplementation on the antioxidant enzymatic system (such as GPx, GR and SOD), GSH and selenium level in liver, kidney, muscle and brain of static magnetic field (SMF) exposed rats. Male adult rats were divided into control rats (n=6), SMF-exposed rats (128 mT; 1h/day for 5 days), selenium-treated rats (Na(2)SeO(3), 0.2mg/l, in drinking water for 4 weeks) and co-exposed rats (selenium for 4 weeks and SMF during the last 5 consecutive days). Sub-acute exposure to SMF induces a decrease of selenium levels in kidney, muscle and brain. Our results also revealed a decrease of GPx activities in kidney and muscle. By contrast, SMF exposure increased total GSH levels and total SOD activities in liver, while glutathione reductase activity is unaffected. Selenium supplementation in SMF-exposed rats restored selenium levels in kidney, muscle and brain and elevated the activities of GPx in kidney and muscle to those of control group. In the liver, selenium supplementation failed to bring down the elevated levels of total GSH and SOD activity. Our investigations suggested that sub-acute exposure to SMF altered the antioxidant response by decreasing the level of total selenium in kidney, muscle and brain. Interestingly, selenium supplementation ameliorates antioxidant capacity in rat tissues exposed to SMF.
本研究旨在探讨硒补充对抗氧化酶系统（如 GPx、GR 和 SOD）、GSH 和肝脏、肾脏、肌肉和大脑中硒水平的影响。雄性成年大鼠分为对照组大鼠（n=6）、静磁场（SMF）暴露组大鼠（128 mT；每天 1 小时，连续 5 天）、硒处理组大鼠（Na2SeO3，0.2mg/l，饮用水中 4 周）和共暴露组大鼠（4 周内补充硒，最后 5 天暴露于 SMF）。亚急性暴露于 SMF 会导致肾脏、肌肉和大脑中的硒水平下降。我们的结果还显示，肾脏和肌肉中的 GPx 活性下降。相比之下，SMF 暴露会增加肝脏中的总 GSH 水平和总 SOD 活性，而谷胱甘肽还原酶活性不受影响。SMF 暴露大鼠补充硒可恢复肾脏、肌肉和大脑中的硒水平，并使肾脏和肌肉中的 GPx 活性恢复到对照组水平。在肝脏中，硒补充未能降低总 GSH 和 SOD 活性的升高水平。我们的研究表明，亚急性暴露于 SMF 通过降低肾脏、肌肉和大脑中总硒水平来改变抗氧化反应。有趣的是，硒补充可改善暴露于 SMF 的大鼠组织的抗氧化能力。
