Major in Food & Nutrition, Mokpo National University, 61 Dorim-ri cheonggye-myeon, Muan-gun, Jeonnam 534-729, Korea.
Nutr Res Pract. 2009 Winter;3(4):259-64. doi: 10.4162/nrp.2009.3.4.259. Epub 2009 Dec 31.
We examined the inhibitory effects of loquat methanol extract on the adhesion, migration, invasion and matrix metalloproteinase (MMP) activities of MDA-MB-231 human breast cancer cell line. Cells were cultured with DMSO or with 10, 25, or 50 microg/ml of loquat methanol extract. Both leaf and seed extracts significantly inhibited growth of MDA-MB-231 cells in a dose-dependent manner, although leaf extract was more effective. Adhesion and migration were significantly inhibited by loquat extracts in a dose-dependent manner. Loquat extract also inhibited the invasion of breast cancer cells in a dose-dependent manner and leaf extract was more effective than seed extract. MMP-2 and MMP-9 activities were also inhibited by loquat extract. Our results indicate that methanol extracts of loquat inhibit the adhesion, migration and invasion of human breast cancer cells partially through the inhibition of MMP activity and leaf extract has more anti-metastatic effects in cell based assay than seed extract. Clinical application of loquat extract as a potent chemopreventive agent may be helpful in limiting breast cancer invasion and metastasis.
我们研究了枇杷甲醇提取物对 MDA-MB-231 人乳腺癌细胞系黏附、迁移、侵袭和基质金属蛋白酶（MMP）活性的抑制作用。细胞用 DMSO 或 10、25 或 50μg/ml 的枇杷甲醇提取物培养。尽管叶提取物的效果更显著，但叶和种子提取物均能显著抑制 MDA-MB-231 细胞的生长，呈剂量依赖性。枇杷提取物能显著抑制细胞黏附和迁移，呈剂量依赖性。枇杷提取物还能抑制乳腺癌细胞的侵袭，呈剂量依赖性，且叶提取物比种提取物的效果更显著。MMP-2 和 MMP-9 的活性也被枇杷提取物抑制。我们的研究结果表明，枇杷甲醇提取物通过抑制 MMP 活性，部分抑制人乳腺癌细胞的黏附、迁移和侵袭，且叶提取物在基于细胞的试验中比种提取物具有更强的抗转移作用。枇杷提取物作为一种有效的化学预防剂的临床应用可能有助于限制乳腺癌的侵袭和转移。
